LIQUID-CHROMATOGRAPHIC SEPARATION OF THE ENANTIOMERS OF METOPROLOL AND ITS GAMMA-HYDROXY METABOLITE ON CHIRALCEL OD FOR DETERMINATION IN PLASMA AND URINE

被引：44

作者：

BALMER, K ^{[1
]}

PERSSON, A ^{[1
]}

LAGERSTROM, PO ^{[1
]}

PERSSON, BA ^{[1
]}

SCHILL, G ^{[1
]}

机构：

[1] UNIV UPPSALA,DEPT ANALYT PHARMACEUT CHEM,S-75123 UPPSALA,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 553卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88509-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The two enantiomers of metoprolol and the four enantiomeric forms of alpha-hydroxymetoprolol were separated by liquid chromatography on a Chiralcel OD column containing a cellulose tris(3,5-dimethylphenylcarbamate) chiral stationary phase. The column efficiency was strongly dependent on the flow-rate and the enantioselectivity was influenced by temperature. Of utmost importance for the chiral separation was the water content of the mobile organic phase. The separation system was used for the separation and determination of the enantiomers in plasma and urine samples. The metoprolol enantiomers could be determined by fluorescence down to 10 nmol/l of each in plasma with a relative standard deviation of less than 15%.

引用

页码：391 / 397

页数：7

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