2,4-dichlorophenoxyacetic acid influx is mediated by an active transport system in Chinese hamster ovary cells

被引：6

作者：

Bergesse, JR ^{[1
]}

Balegno, HF ^{[1
]}

机构：

[1] UNIV NACL RIO CUARTO,DEPT BIOL MOLEC,RA-5800 RIO CUARTO,CORDOBA,ARGENTINA

来源：

TOXICOLOGY LETTERS | 1995年 / 81卷 / 2-3期

关键词：

2,4-dichlorophenoxyacetic acid; influx; Chinese hamster ovary cells;

D O I：

10.1016/0378-4274(95)03428-5

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The influx of 2,4-dichlorophenoxyacetic acid (2,4-D) into Chinese hamster ovary (CHO) cells was studied. The cells mainly took up but did not metabolize the undissociated form of the herbicide. The uptake of 2,4-D was carried out against a concentration gradient and was inhibited by sodium azide and dinitrophenol. The results presented here show that the herbicide influx was an active, energy dependent process. (Na+ + K+)ATPase does not seem to be involved because ouabain, an inhibitor of the enzyme, did not affect the 2,4-D uptake.

引用

页码：167 / 173

页数：7

共 14 条

[1]

Sinton, Fan, Erickson, Lee, Biodegradation of 2,4-D and related xenobiotic compounds, Enzyme Microb. Technol., 8, 7, pp. 395-403, (1986)

[2]

Rivarola, Bergesse, Balegno, DNA and protein synthesis inhibition in Chinese Hamster Ovary Cells by dichlorophenoxyacetic acid, Toxicol. Lett., 29, pp. 137-144, (1985)

[3]

Bergesse, Rivarola, Balegno, Changes in 2-aminoisobutyric acid and cycloleucine uptake produced by 2,4-dichlorophenoxyacetic acid in Chinese Hamster Ovary cells, Toxicol. Lett., 36, pp. 189-196, (1987)

[4]

Ham, McKeehan, Media and growth requirements, Methods in Enzymology, 58, (1979)

[5]

Shipman, Trypsin A: mammalian tissues, Tissue Culture Methods and Applications, (1973)

[6]

Liebennan, Raben, Whittenberger, Glaser, Effect of plasma membranes on solute transport in 3T3 cells, J. Biol. Chem., 254, pp. 6357-6361, (1979)

[7]

Kelley, Potter, Regulation of amino acid transport systems by amino acid depletion and supplementation in monolayer cultures of rat hepatocytes, J. Biol. Chem., 253, pp. 9009-9017, (1978)

[8]

Kletzien, Pariza, Becker, Potter, A method using 3-O-methyl-D-glucose and phloretin for the determination of intracellular water space of cells in monolayer culture, Anal. Biochem., 68, pp. 537-544, (1975)

[9]

Erne, Determination of phenoxyacetic herbicide residues in biological materials, Acta Vet. Scand., 7, pp. 77-96, (1966)

[10]

Jhonsen, Stokke, Prydz, Hela cell plasma membranes I. 5′ nucleotidase and ouabainsensitive ATPase as markers of plasma membranes, J. Cell Biol., 63, pp. 357-363, (1974)

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