SOLID-PHASE EXTRACTION OF ASCORBIC-ACID 2-SULFATE FROM CYSTS OF THE BRINE SHRIMP ARTEMIA-FRANCISCANA

被引：11
|
作者：
NELIS, HJ [1 ]
MERCHIE, G [1 ]
LAVENS, P [1 ]
SORGELOOS, P [1 ]
DELEENHEER, AP [1 ]
机构：
[1] STATE UNIV GHENT,MED BIOCHEM & KLIN ANALYSE LAB,B-9000 GHENT,BELGIUM
来源：
ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 08期
关键词：
D O I：
10.1021/ac00080a019
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Two procedures are described for the solid-phase extraction of ascorbic acid 2-sulfate from cysts of the brine shrimp Artemia, The first one is based on reversed-phase ion pair extraction on an octadecylsilica cartridge using tetrabutylammonium as the counterion, followed by elution with methanol. In the second procedure ascorbic acid 2-sulfate is retained on a DEAE silica cartridge and eluted with 0.1 M sodium salicylate. Both approaches were used as part of a quantitative reversed-phase ion pair liquid chromatographic method with detection at 254 nm. The anion exchange extraction procedure is superior to its ion pair counterpart in terms of recovery (88.7 +/- 3.5% versus 63.0 +/- 12.7%), reproducibility (CVs of 3.6-7.7% versus 6.3-18.7%), and simplicity.
引用
收藏
页码：1330 / 1333
页数：4
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