Development of a Four-way Interface for Online Capillary Isoelectric Focusing-Electrospray-Mass Spectrometry (CIEF-ESI-MS)

被引：0
|
作者：
Yu, Hai Dong [1 ,2 ]
Kim, Byungjoo [1 ]
Shin, Dae-Ho [3 ]
Ahn, Seonghee [1 ]
机构：
[1] Korea Res Inst Stand & Sci, Div Metrol Qual Life, Yuseong 305600, Daejeon, South Korea
[2] Univ Sci & Technol, Dept Bioanalyt Sci, Yuseong 305333, Daejeon, South Korea
[3] Bio Med Technol Ltd, Seoul 100894, South Korea
来源：
MASS SPECTROMETRY LETTERS | 2013年 / 4卷 / 04期
关键词：
CIEF-ESI-MS; Interface; Carbonic anhydrase I; Fourier transform mass spectrometry;
D O I：
10.5478/MSL.2013.4.4.83
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A new interface for coupling CIEF and MS using a four-way cross has been developed in a single mechanical system. This new interface could be operated without the electric discontinuity and reinstallation of lines. Additionally, a bare fused silica capillary was facilitated as a spray needle to produce electrospray and to guide catholyte or sheath liquid. Focusing for CIEF was completed in a hanging droplet at the end of spray needle. This capillary spray needle also provided stable spray, enhanced the ionization efficiency and increased sensitivity. Results with carbonic anhydrase I showed that focusing and spraying were well completed with the new interface and the new spray needle.
引用
收藏
页码：83 / 86
页数：4
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