FIELD PERFORMANCE OF ELITE TRANSGENIC MAIZE PLANTS EXPRESSING AN INSECTICIDAL PROTEIN DERIVED FROM BACILLUS-THURINGIENSIS

被引：651

作者：

KOZIEL, MG ^{[1
]}

BELAND, GL ^{[1
]}

BOWMAN, C ^{[1
]}

CAROZZI, NB ^{[1
]}

CRENSHAW, R ^{[1
]}

CROSSLAND, L ^{[1
]}

DAWSON, J ^{[1
]}

DESAI, N ^{[1
]}

HILL, M ^{[1
]}

KADWELL, S ^{[1
]}

LAUNIS, K ^{[1
]}

LEWIS, K ^{[1
]}

MADDOX, D ^{[1
]}

MCPHERSON, K ^{[1
]}

MEGHJI, MR ^{[1
]}

MERLIN, E ^{[1
]}

RHODES, R ^{[1
]}

WARREN, GW ^{[1
]}

WRIGHT, M ^{[1
]}

EVOLA, SV ^{[1
]}

机构：

[1] CIBA GEIGY SEEDS,BLOOMINGTON,IL 61702

来源：

BIO-TECHNOLOGY | 1993年 / 11卷 / 02期

关键词：

D O I：

10.1038/nbt0293-194

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We introduced a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis into immature embryos of an elite line of maize using microprojectile bombardment. This gene was expressed using either the CaMV 35S promoter or a combination of two tissue specific promoters derived from maize. High levels of CryIA(b) protein were obtained using both promoter configurations. Hybrid maize plants resulting from crosses of transgenic elite inbred plants with commercial inbred lines were evaluated for resistance to European corn borer under field conditions. Plants expressing high levels of the insecticidal protein exhibited excellent resistance to repeated heavy infestations of this pest.

引用

页码：194 / 200

页数：7

共 35 条

[1] BACILLUS-THURINGIENSIS DELTA-ENDOTOXIN EXPRESSED IN TRANSGENIC NICOTIANA-TABACUM PROVIDES RESISTANCE TO LEPIDOPTERAN INSECTS [J].