IDENTIFICATION OF CYSTIC-FIBROSIS PROTEIN AS A COMPLEX OF 2 CALCIUM-BINDING PROTEINS PRESENT IN HUMAN-CELLS OF MYELOID ORIGIN

被引：40

作者：

BARTHE, C

FIGARELLA, C

CARRERE, J

GUYCROTTE, O

机构：

[1] GRP RECH GLANDES EXOCRINES, 27 BD LEI ROURE, BP 156, F-13273 MARSEILLE 09, FRANCE

[2] HOP RENEE SABRAN, CERM, HYERES, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1096卷 / 02期

关键词：

CYSTIC FIBROSIS PROTEIN; SERUM; IMMUNOLOGICAL RELATIONSHIP;

D O I：

10.1016/0925-4439(91)90057-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cystic fibrosis protein is a serum protein characterized by a pI close to 8.4 and present with a higher concentration in serum and plasma of cystic fibrosis carriers than in controls. This protein was found immunologically indistinguishable from the cystic fibrosis antigen isolated from granulocytes and presenting a sequence analogous to that of MRP-8, a calcium-binding protein expressed in the myeloid cell lineage. Using antibodies directed against MRP-8 and its closely associated calcium-binding protein, MRP-14, we demonstrate here that cystic fibrosis protein purified from serum is a complex of the two proteins MRP-8 and MRP-14.

引用

页码：175 / 177

页数：3

共 14 条

[1] CORRELATION BETWEEN THE CYSTIC-FIBROSIS PROTEIN DETECTED BY ISOELECTRIC-FOCUSING AND A PROTEIN OF 12 KDA DETECTED BY SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS [J].