IDENTIFICATION OF A NOVEL NUCLEOSIDE, 1,N-6-DIMETHYLADENOSINE, IN HUMAN CANCER URINE

被引:7
作者
HAMMARGREN, WM [1 ]
SCHRAM, KH [1 ]
NAKANO, K [1 ]
YASAKA, T [1 ]
机构
[1] PL COMPREHENS RES INST, TONDABAYASHI, OSAKA 584, JAPAN
基金
美国国家卫生研究院;
关键词
GAS CHROMATOGRAPHY; MASS SPECTROMETRY; DIMETHYLADENOSINE; URINE;
D O I
10.1016/S0003-2670(00)83814-3
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The identification of 1,N6-dimethyladenosine in the urine of a lung cancer patient is reported. Prior to the characterization of the trimethylsilyl derivative by gas chromatography-mass spectrometry, the separation and partial purification of the compound was accomplished by a boronate gel affinity technique followed by reversed-phase high-performance liquid chromatography. The structure was confirmed by comparison of the mass spectral and chromatographic features of the urinary component to those of a synthetic standard.
引用
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页码:201 / 209
页数:9
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