GAS CHROMATOGRAPHY;
MASS SPECTROMETRY;
DIMETHYLADENOSINE;
URINE;
D O I:
10.1016/S0003-2670(00)83814-3
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
The identification of 1,N6-dimethyladenosine in the urine of a lung cancer patient is reported. Prior to the characterization of the trimethylsilyl derivative by gas chromatography-mass spectrometry, the separation and partial purification of the compound was accomplished by a boronate gel affinity technique followed by reversed-phase high-performance liquid chromatography. The structure was confirmed by comparison of the mass spectral and chromatographic features of the urinary component to those of a synthetic standard.