Two-Step Chromatographic Method for the Separation and Purification of Recombinant Angiogenesis Inhibitor Kringle 5

被引：0

作者：

Jia Xingui ^{[1
]}

Bian Liujiao ^{[1
]}

机构：

[1] Northwest Univ, Coll Life Sci, Natl Engn Res Ctr Miniaturized Detect Syst, Xian 710069, Shaanxi, Peoples R China

来源：

CHINESE JOURNAL OF CHROMATOGRAPHY | 2007年 / 25卷 / 03期

关键词：

affinity column chromatography; gel exclusion column chromatography; recombinant angiogenesis inhibitor Kringle 5; separation; purification;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The Kringle 5 domain of plasminogen, which was previously shown to inhibit angiogenesis in vitro and in vivo, is one of the most potent angiogenesis inhibitors known to date. A two-step chromatographic method, which consists of Chelating Sepharose Fast Flow chelated with Ni2+ affinity medium and Sephadex G-75 medium, was established to separate and purify recombinant angiogenesis inhibitor Kringle 5 (rK5). Through the two-step chromatographic purification process, the obtained rK5 was confirmed to be homogeneous on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and its relative molecular mass was estimated to be about 32 000, which matched well with the prediction by gene sequence. Its purity was about 98%, and the total protein recovery of this method was 0.63%. In addition, it was found that it inhibited the blood vessel growth of chick embryo chorioallantoic membrane effectively.

引用

页码：344 / 347

页数：4

共 12 条

[1] Enhanced anti-angiogenic effect of a deletion mutant plasminogen kringle 5 on of neovascularization [J].