FUNCTIONAL AND BIOCHEMICAL CHARACTERISTICS OF A SOLUBLE B-LYMPHOCYTE PROLIFERATION-INHIBITING ACTIVITY PRODUCED BY BONE-MARROW CELLS FROM MULTIPLE-MYELOMA PATIENTS

被引：4
作者：
QUESADA, S [1 ]
LEO, R [1 ]
DEICHER, H [1 ]
PEEST, D [1 ]
机构：
[1] HANNOVER MED SCH,IMMUNOL & TRANSFUS MED ABT,HANNOVER,GERMANY
来源：
CELLULAR IMMUNOLOGY | 1995年 / 162卷 / 02期
关键词：
D O I：
10.1006/cimm.1995.1079
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Several mechanisms are discussed as inducers for polyclonal hypogammaglobulinemia in multiple myeloma. A soluble noncytotoxic activity which inhibits in vitro the proliferation of normal polyclonal spleen B lymphocytes was measured in the supernatant of cultured bone marrow mononuclear cells from multiple myeloma patients, In addition, human B lymphoblastic cell lines (CESS, Daudi) and human T lymphocytes were sensitive to the antiproliferative effect of the suppressor activity, while other cell lines (RPMI 8226, IM9, CTL6, L1210, HL-60, and K562) were not. The activity was detected in a 5-kDa fraction and was stable to heating (30 min, 56 degrees C) and proteolytic enzymes. Extraction experiments using chloroform:methanol (2:1) suggest a lipid character of the suppressor activity. (C) 1995 Academic Press, Inc.
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页码：275 / 281
页数：7
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