SPERMIATION OF COMMON TENCH (TINCA-TINCA L) STIMULATED WITH INJECTION OR IMPLANTATION OF GNRH ANALOGS AND INJECTION OF CARP PITUITARY EXTRACT

被引：22

作者：

LINHART, O

PETER, RE

ROTHBARD, S

ZOHAR, Y

KVASNICKA, P

机构：

[1] RES INST FISH CULTURE & HYDROBIOL,DEPT FISH GENET & BREEDING,CS-38925 VODNANY,CZECH REPUBLIC

[2] UNIV ALBERTA,DEPT ZOOL,EDMONTON,AB T6G 2E9,CANADA

[3] FISH BREEDING CTR,YAFIT LAB,IL-38810 GAN SHMUEL,ISRAEL

[4] UNIV MARYLAND,CTR MARINE BIOTECHNOL,BALTIMORE,MD 21202

来源：

AQUACULTURE | 1995年 / 129卷 / 1-4期

关键词：

TINCA TINCA; SPERMIATION; GNRH ANALOGS; CARP PITUITARY EXTRACT;

D O I：

10.1016/0044-8486(94)00233-E

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Spermiation was stimulated in the common tench, Tinca tinca L., by injection of carp pituitary extract (CPE; 0.5 mg.kg(-1) body weight), carp homogenized pituitary (1 mg.kg(-1) body weight), [D-Arg(6),Pro(9)-NHEt] salmon GnRH (20 mu g.kg(-1) body weight) and [D-Ala(6),Pro-NHEt] mammalian GnRH slow-releasing implants at a dose of 25 mu g per male (average, 652 g of body weight), The sperm was collected daily for 5 days post-stimulation and diluted with immobilizing solution (180 mM NaCl, 20 mM Tris, pH 7). Sperm density, expressed as billions per kg of body weight, was 47.31 for CPE, 49.42 for carp homogenized pituitary, 51.84 for (D-Arg(6),Pro(9)-NHEt] salmon GnRH, 28.93 for GnRH analogue implant and 13.73 for the control group treated with Ringer solution. The total number of spermatozoa obtained by stimulation with [D-Arg(6),Pro(9)-NHEt] salmon GnRH was significantly higher (P < 0.05) than for the carp-hypophysis-treated groups. The best spermiation with the GnRH analogue implantant group was at 4 days after implantation (P < 0.001).

引用

页码：119 / 121

页数：3