MODULATION OF INTERLEUKIN-1 RECEPTOR EXPRESSION BY TRANSFORMING GROWTH-FACTOR-BETA IN CULTURED RABBIT ARTICULAR CHONDROCYTES - ANALYSIS BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN-REACTION

被引：12

作者：

PRONOST, S ^{[1
]}

SEGOND, N ^{[1
]}

MACRO, M ^{[1
]}

REDINI, F ^{[1
]}

PENFORNIS, H ^{[1
]}

JULLIENNE, A ^{[1
]}

MOUKHTAR, MS ^{[1
]}

PUJOL, JP ^{[1
]}

机构：

[1] FAC MED CAEN,INSERM,CJF 9106,BIOCHIM TISSU CONJONCTIF LAB,F-14032 CAEN,FRANCE

来源：

OSTEOARTHRITIS AND CARTILAGE | 1995年 / 3卷 / 03期

关键词：

REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION; TRANSFORMING GROWTH FACTOR-BETA; INTERLEUKIN-1 RECEPTOR TYPE I; RABBIT ARTICULAR CHONDROCYTES;

D O I：

10.1016/S1063-4584(05)80049-4

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

摘要：

Interleukin-1 receptor type I(IL-1RI) expression in cultured rabbit articular chondrocytes (RAC) was studied by reverse transcription-polymerase chain reaction (RT-PCR). A cDNA probe specific for the rabbit IL-1RI gene was constructed using primers derived from the sequence data of the human, murine and chick receptors. Transforming growth factor-beta 1 (TGF beta-1) was shown to transiently increase the level of expected 900-bp PCR product at 1 h of incubation and decrease the expression at 48 and 72 h with no effect at 24 h. In receptor binding assays using [I-125]-IL-1 alpha, TGF beta decreased IL-1R bioactivity at all time points. These results suggest that TGF beta-induced down-regulation of IL-1 RI could be responsible for its ability to antagonize the effect of IL-1 and that TGF beta may have a role in the repair of articular cartilage.

引用

页码：147 / 155

页数：9

共 35 条

[1]

Pujol, Loyau, Interleukin-1 and osteoarthritis, Life Sci, 41, pp. 1187-1198, (1987)

[2]

Saklatvala, Regulation of chondrocytes by cytokines, Biological regulation of the chondrocytes, pp. 191-204, (1992)

[3]

Gowen, Wood, Ihrie, Meats, Russell, Stimulation by human interleukin 1 of cartilage breakdown and production of collagenase and proteoglycanase by human chondrocytes but not by human osteoblats in vitro, Biochim Biophys Acta, 797, pp. 186-193, (1984)

[4]

Saklatvala, Sarsfield, Townsend, Pig interleukin-1. Purification of two immunologically different leukocyte proteins that cause cartilage resorption, lymphocyte activation and fever, J Exp Med, 162, pp. 1208-1222, (1985)

[5]

Pujol, Brisset, Jourdan, Et al., Effect of a mononuclear cell factor (MCF) on collagen production in cultured articular chondrocytes. Role of prostaglandin E2, Biochem Biophys Res Commun, 119, pp. 499-508, (1984)

[6]

Benton, Tyler, Inhibition of cartilage proteoglycan synthesis by interleukin-1, Biochem Biophys Res Commun, 154, pp. 421-428, (1988)

[7]

Mankin, The reaction of articular cartilage to injury and osteoarthritis (Part I), N Engl J Med, 291, pp. 1285-1309, (1974)

[8]

Mankin, The reaction of articular cartilage to injury and osteoarthritis (Part II), N Engl J Med, 291, pp. 1310-1335, (1974)

[9]

Dower, Urdal, The interleukin-1 receptor, Immunology Today, 8, pp. 46-51, (1987)

[10]

Dower, Sims, Ceretti, Bird, The interleukin-1 system receptors ligands and signals, Chem Immunol, 51, pp. 33-64, (1991)

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