NONRADIOMETRIC DETECTION OF DNA CROSS-LINKS IN MUSSEL HEMOLYMPH BY ALKALINE ELUTION

被引：17
作者：
BATEL, R
VUKMIROVIC, M
BIHARI, N
ZAHN, RK
MULLER, WEG
机构：
[1] ACAD SCI & LITERATURE MAINZ, COMMISS MOLEC BIOL, MAINZ, GERMANY
[2] UNIV MAINZ, INST PHYSIOL CHEM, W-6500 MAINZ, GERMANY
来源：
ANALYTICAL BIOCHEMISTRY | 1993年 / 212卷 / 02期
关键词：
D O I：
10.1006/abio.1993.1347
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of bleomycin-Fe(II) complex in the determination of DNA crosslinks by alkaline elution was investigated for the purpose of developing a simple nonradiometric method applicable for detection of DNA damage in marine organisms. The assay involves preparation of active bleomycin-Fe(II) complex and its application in the washing step of the original alkaline elution procedure. Treatment of cell-free DNA from Friend leukemia cells and mussel hemolymph with bleomycin-Fe(II) complex directly on the filter resulted in a concentration-dependent strand scission response with excellent correlation (r = 0.94 and 0.98, respectively). The procedure was validated by determination of DNA crosslinks in mussels injected with mitomycine C and formaldehyde. © 1994 Academic Press, Inc. All rights reserved.
引用
收藏
页码：402 / 406
页数：5
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