BY55 MONOCLONAL-ANTIBODY DELINEATES WITHIN HUMAN CORD-BLOOD AND BONE-MARROW LYMPHOCYTES DISTINCT CELL SUBSETS MEDIATING CYTOTOXIC ACTIVITY

被引：47

作者：

BENSUSSAN, A ^{[1
]}

GLUCKMAN, E ^{[1
]}

ELMARSAFY, S ^{[1
]}

SCHIAVON, V ^{[1
]}

MANSUR, IG ^{[1
]}

DAUSSET, J ^{[1
]}

BOUMSELL, L ^{[1
]}

CAROSELLA, E ^{[1
]}

机构：

[1] HOP ST LOUIS, CEA, IMMUNORADIOBIOL LAB, UNITE RECH BIOL CELLULES SOUCHES, F-75475 PARIS 10, FRANCE

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 19期

关键词：

CYTOTOXIC LYMPHOCYTE SUBSET; CORD BLOOD LYMPHOCYTES;

D O I：

10.1073/pnas.91.19.9136

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We had previously reported, using BY55 monoclonal antibody, a cell surface 80-kDa protein restricted to human functional peripheral blood cytotoxic lymphocytes with either natural killer CD3(-) or cytotoxic T lymphocyte CD3(+)CD8(+) phenotype. In the present report, we studied the cytotoxic lymphocytes in adult bone marrow and newborn cord blood as these organs are commonly used as sources of hematological stem cells for allogeneic transplantation. Our results showed that BY55 mAb labeled only 5-10% of the bone marrow lymphocytes, which included a major proportion of CD3(+)CD8(+) cytotoxic T lymphocytes. Interestingly, within cord blood cells, BY55(+) lymphocytes represented 20-35% of the lymphocytes corresponding exclusively to a CD3(-) cell subset. Furthermore, we detected in cord blood no cytotoxic T lymphocyte activity but we demonstrated that the CD3(-)BY55(+) cell subset contained the whole natural killer activity.

引用

页码：9136 / 9140

页数：5

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