The technological, microbiological, and sensory storage characteristics of low temperature sous vide cooked roast beef were investigated. The effect of two heat treatments, 59 degrees C (P(70)(10)in core 8.4) and 62 degrees C (P(70)(10)in core 15.9) on the stability of spiced roast beef made from Musculus semitendinosus with a high initial microbial load were compared as well as storage temperatures of 2 and 10 degrees C. Although chilling baths with circulating water were used, recommended chilling rates for sous vide products could not be attained. Yield was significantly higher at 59 degrees C and at a storage temperature of 2 degrees C but decreased during storage. At 62 degrees C the meat became significantly more tender than at 59 degrees C as measured by shear force. No differences in microbiology were observed between heating regimes. At low storage temperature products were microbiologically stable over a 35-day period. At 10 degrees C, however, a rise in psychrotrophic aerobic counts and occasional pack swelling was observed. In a commercial scale experiment conducted with sous vide cooked (62 degrees C) beef with low initial counts, no increase in aerobic counts was observed at 2, 5 and 10 degrees C while swelling occurred in 28% of the packages stored at 10 degrees C and in none at 2 and 5 degrees C. The swelling was due to different types of gas-producing clostridia. Warmed-over flavour (as TEARS) showed no development during storage in intact packages, while slicing and serving the roast beef under commercial conditions resulted in a marked increase to > 100 mu mole kg(-1). In spiced roast beef only minor changes in off-odour and off-flavour of the product were observed during 23 days of storage at 2 degrees C.