CRYSTAL-STRUCTURE OF ACTIVE ELONGATION-FACTOR TU REVEALS MAJOR DOMAIN REARRANGEMENTS

被引：507
作者：
BERCHTOLD, H
RESHETNIKOVA, L
REISER, COA
SCHIRMER, NK
SPRINZL, M
HILGENFELD, R
机构：
[1] HOECHST AKT GESELL,CENT RES G865A,PROT CRYSTALLOG,D-65926 FRANKFURT,GERMANY
[2] UNIV BAYREUTH,BIOCHEM LAB,D-95440 BAYREUTH,GERMANY
[3] VA ENGELHARDT MOLEC BIOL INST,MOSCOW 117984,RUSSIA
来源：
NATURE | 1993年 / 365卷 / 6442期
关键词：
D O I：
10.1038/365126a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The crystal structure of intact elongation factor Tu (EF-Tu) from Thermus thermophilus has been determined and refined at an effective resolution of 1.7 angstrom, with incorporation of data extending to 1.45 angstrom. The effector region, including interaction sites for the ribosome and for transfer RNA, is well defined. Molecular mechanisms are proposed for transduction and amplification of the signal induced by GTP binding as well as for the intrinsic and effector-enhanced GTPase activity of EF-Tu. Comparison of the structure with that of EF-Tu-GDP reveals major mutual rearrangements of the three domains of the molecule.
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页码：126 / 132
页数：7
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共 50 条
[1]   NEW ANTIBIOTIC THAT ACTS SPECIFICALLY ON THE GTP-BOUND FORM OF ELONGATION FACTOR-TU [J].
ANBORGH, PH ;
PARMEGGIANI, A .
EMBO JOURNAL, 1991, 10 (04) :779-784
[2]   STUDIES ON POLYPEPTIDE-CHAIN-ELONGATION FACTORS FROM AN EXTREME THERMOPHILE, THERMUS-THERMOPHILUS-HB8 .2. CATALYTIC PROPERTIES [J].
ARAI, KI ;
ARAI, N ;
NAKAMURA, S ;
OSHIMA, T ;
KAZIRO, Y .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1978, 92 (02) :521-531
[3]   CALCULATION OF AN OMIT MAP [J].
BHAT, TN .
JOURNAL OF APPLIED CRYSTALLOGRAPHY, 1988, 21 :279-281
[4]   BACTERIOPHAGE QBETA REPLICASE CONTAINS PROTEIN-BIOSYNTHESIS ELONGATION FACTORS EF TU AND EF TS [J].
BLUMENTHAL, T ;
WEBER, K ;
LANDERS, TA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (05) :1313-+
[5]  
BOURNE HR, 1991, NATURE, V349, P117, DOI 10.1038/349117a0
[6]   THE GTPASE SUPERFAMILY - A CONSERVED SWITCH FOR DIVERSE CELL FUNCTIONS [J].
BOURNE, HR ;
SANDERS, DA ;
MCCORMICK, F .
NATURE, 1990, 348 (6297) :125-132
[7]   FREE R-VALUE - A NOVEL STATISTICAL QUANTITY FOR ASSESSING THE ACCURACY OF CRYSTAL-STRUCTURES [J].
BRUNGER, AT .
NATURE, 1992, 355 (6359) :472-475
[8]   SLOW-COOLING PROTOCOLS FOR CRYSTALLOGRAPHIC REFINEMENT BY SIMULATED ANNEALING [J].
BRUNGER, AT ;
KRUKOWSKI, A ;
ERICKSON, JW .
ACTA CRYSTALLOGRAPHICA SECTION A, 1990, 46 :585-593
[9]   EXTENSION OF MOLECULAR REPLACEMENT - A NEW SEARCH STRATEGY BASED ON PATTERSON CORRELATION REFINEMENT [J].
BRUNGER, AT .
ACTA CRYSTALLOGRAPHICA SECTION A, 1990, 46 :46-57
[10]   CRYSTALLOGRAPHIC R-FACTOR REFINEMENT BY MOLECULAR-DYNAMICS [J].
BRUNGER, AT ;
KURIYAN, J ;
KARPLUS, M .
SCIENCE, 1987, 235 (4787) :458-460
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