PHYLOGENETIC AMPLIFICATION FOR THE DETECTION OF UNCULTURED BACTERIA AND THE ANALYSIS OF COMPLEX MICROBIOTA

被引：23

作者：

GOBEL, UB

机构：

[1] Institut für Mikrobiologie und Hygiene, Medizinische Fakultät (Charité) der Humboldt-Universität Berlin, D-10117 Berlin

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1995年 / 23卷 / 01期

关键词：

16S RIBOSOMAL-RNA; IN VITRO AMPLIFICATION; PCR; PROBE; IN SITU HYBRIDIZATION; UNIVERSAL PRIMER; UNCULTURED BACTERIA;

D O I：

10.1016/0167-7012(95)00025-G

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Comparative rRNA sequence analysis combined with in vitro amplification and nucleic acid hybridization procedures deeply affects our understanding of environmental, medical and veterinary microbiology. It is now not only possible to study cultivable microorganisms, but to identify yet uncultured or truly uncultivable microorganisms. The true extent of microbial diversity, which has been largely underestimated by culture-based isolation and characterization procedures, can finally be determined.

引用

页码：117 / 128

页数：12

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