A GENERAL PURIFICATION PROCEDURE FOR CHEMICALLY SYNTHESIZED OLIGORIBONUCLEOTIDES

被引：53

作者：

MURRAY, JB ^{[1
]}

COLLIER, AK ^{[1
]}

ARNOLD, JRP ^{[1
]}

机构：

[1] UNIV LEEDS, SCH CHEM, LEEDS LS2 9JT, W YORKSHIRE, ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1994年 / 218卷 / 01期

基金：

英国惠康基金;

关键词：

D O I：

10.1006/abio.1994.1157

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A general procedure for the purification of chemically synthesized oligoribonucleotides is reported. Purification based on the use of a single reverse-phase HPLC column with buffer systems of differing ion-pairing capacity is described. These methods have been applied to the preparation of a series of RNAs which range in size from 10 to 46 nucleotides. The yields obtained are high, up to 53% (based on isolated product compared to those obtained from final trityl assay). The purity of the isolated material is 96-99%. Thus with this general procedure, milligram quantities of extremely pure RNA can be efficiently obtained. (C) 1994 Academic Press, Inc.

引用

页码：177 / 184

页数：8

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