PRODUCTION IN ESCHERICHIA-COLI AND ONE-STEP PURIFICATION OF BIFUNCTIONAL HYBRID PROTEINS WHICH BIND MALTOSE - EXPORT OF THE KLENOW POLYMERASE INTO THE PERIPLASMIC SPACE

被引：114

作者：

BEDOUELLE, H ^{[1
]}

DUPLAY, P ^{[1
]}

机构：

[1] INST PASTEUR, UNITE PROGRAMMAT MOLEC & TOXICOL GENET,CNRS,U271, INSERM,U163, F-75724 PARIS 15, FRANCE

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1988年 / 171卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1988.tb13823.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：541 / 549

页数：9

共 44 条

[11] SILENT AND FUNCTIONAL-CHANGES IN THE PERIPLASMIC MALTOSE-BINDING PROTEIN OF ESCHERICHIA-COLI-K12 .1. TRANSPORT OF MALTOSE [J].

DUPLAY, P ;

SZMELCMAN, S ;

BEDOUELLE, H ;

HOFNUNG, M .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 194 (04) :663-673

[12]

DUPLAY P, 1984, J BIOL CHEM, V259, P606

[13]

EMR SD, 1982, J BIOL CHEM, V257, P5852

[14] AFFINITY CHROMATOGRAPHIC ISOLATION OF PERIPLASMIC MALTOSE BINDING-PROTEIN OF ESCHERICHIA-COLI [J].

FERENCI, T ;

KLOTZ, U .

FEBS LETTERS, 1978, 94 (02) :213-217

[15] RAPID PURIFICATION OF A CLONED GENE-PRODUCT BY GENETIC FUSION AND SITE-SPECIFIC PROTEOLYSIS [J].

GERMINO, J ;

BASTIA, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (15) :4692-4696

[16] PRODUCTION OF ABNORMAL PROTEINS IN ESCHERICHIA-COLI STIMULATES TRANSCRIPTION OF ION AND OTHER HEAT-SHOCK GENES [J].

GOFF, SA ;

GOLDBERG, AL .

CELL, 1985, 41 (02) :587-595

[17] STUDIES ON TRANSFORMATION OF ESCHERICHIA-COLI WITH PLASMIDS [J].

HANAHAN, D .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (04) :557-580

[18]

HEINS JN, 1966, PROCEDURES NUCLEIC A, V1, P79

[19]

ITO K, 1982, J BIOL CHEM, V257, P9895

[20] CONSTRUCTION OF A PLASMID THAT OVERPRODUCES THE LARGE PROTEOLYTIC FRAGMENT (KLENOW FRAGMENT) OF DNA-POLYMERASE-I OF ESCHERICHIA-COLI [J].

JOYCE, CM ;

GRINDLEY, NDF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (07) :1830-1834

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