AUTOCRINE INHIBITION OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR BY INTRACELLULAR EXPRESSION OF A SINGLE-CHAIN ANTIBODY

被引：41
作者：
BEERLI, RR
WELS, W
HYNES, NE
机构：
[1] FRIEDRICH MIESCHER INST,CH-4002 BASEL,SWITZERLAND
[2] TUMOR BIOL CTR,D-79106 FREIBURG,GERMANY
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 204卷 / 02期
关键词：
D O I：
10.1006/bbrc.1994.2511
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A gene encoding a single-chain antibody which specifically binds the human epidermal growth factor (EGF) receptor has been constructed and expressed intracellularly. The single-chain antibody is derived from monoclonal antibody 225 which competes with EGF for binding to the extracellular domain of the receptor. The single-chain antibody was provided with a signal peptide to direct it to the secretory pathway and was expressed in EGF receptor transformed NIH/3T3 fibroblasts. EGF induced activation of its receptor was reduced in these cells. In addition, EGF-induced anchorage-independent growth of the cells was inhibited. The data suggest that the single-chain antibody functions in an autocrine fashion to inhibit the activity of the EGF receptor. (C) 1994 Academic Press, Inc.
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页码：666 / 672
页数：7
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