A 22-AMINO-ACID PEPTIDE RESTORES DNA-BINDING ACTIVITY TO DIMERIZATION-DEFECTIVE MUTANTS OF THE ESTROGEN-RECEPTOR

被引：81

作者：

LEES, JA ^{[1
]}

FAWELL, SE ^{[1
]}

WHITE, R ^{[1
]}

PARKER, MG ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,MOLEC ENDOCRINOL LAB,LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 10期

关键词：

D O I：

10.1128/MCB.10.10.5529

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified residues within the estrogen receptor that are required for dimerization and high-affinity DNA binding. A 22-amino-acid peptide encompassing these residues was sufficient to restore DNA-binding activity to a mutant receptor lacking most of the hormone-binding domain. Point mutagenesis of the fusion protein confirmed that this sequence continued to mediate dimerization in a manner similar to that within the native receptor, although its position relative to the DNA-binding domain was appreciably altered.

引用

页码：5529 / 5531

页数：3

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