REFINED PHYSICAL MAPPING OF THE SEC-1 LOCUS ON THE SATELLITE OF CHROMOSOME 1R OF RYE (SECALE-CEREALE)

被引：20

作者：

BUSCH, W ^{[1
]}

HERRMANN, RG ^{[1
]}

MARTIN, R ^{[1
]}

机构：

[1] UNIV MUNICH,INST BOT,D-80638 MUNICH,GERMANY

来源：

GENOME | 1995年 / 38卷 / 05期

关键词：

FLUORESCENCE IN SITU HYBRIDIZATION; PHYSICAL MAPPING; GENETIC MAPPING; SECALIN; RYE; B-HORDEIN; RDNA;

D O I：

10.1139/g95-117

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Sec-1 locus (omega-secalin) of rye (Secale cereale L.) was mapped in the satellite of the short arm of chromosome 1R using fluorescence in situ hybridization and a genomic probe called pSec2B. Sec-1 is located in the middle of the satellite at the junction of the proximal euchromatic and the distal heterochromatic regions. Double hybridization experiments using rDNA and pSec2B showed that the NOR spans over the secondary constriction of the short arm of chromosome 1R and that there is a clearly visible gap between the NOR and Sec-1. Heterologous hybridization of pSec2B to barley visualized the B-hordein locus on chromosome 1H.

引用

页码：889 / 893

页数：5

共 31 条

[1] A CYTOGENETIC MAP ON THE ENTIRE LENGTH OF RYE CHROMOSOME-1R, INCLUDING ONE TRANSLOCATION BREAKPOINT, 3 ISOZYME LOCI AND 4 C-BANDS [J].