WATER ACTIVITY DOES NOT INFLUENCE THE ENANTIOSELECTIVITY OF LIPASE PS AND LIPOPROTEIN-LIPASE IN ORGANIC-SOLVENTS

被引：55

作者：

BOVARA, R ^{[1
]}

CARREA, G ^{[1
]}

OTTOLINA, G ^{[1
]}

RIVA, S ^{[1
]}

机构：

[1] CNR,IST CHIM ORMONI,VIA MARIO BIANCO 9,I-20131 MILAN,ITALY

来源：

BIOTECHNOLOGY LETTERS | 1993年 / 15卷 / 02期

关键词：

D O I：

10.1007/BF00133018

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The activity and enantioselectivity of Lipase PS from Pseudomonas cepacia and lipoprotein lipase from Pseudomonas sp. were investigated in organic solvents preequilibrated to water activities ranging from <0.1 to 0.53, using as a model reaction the transesterification between (+/-)-sulcatol and vinyl acetate. Variations of water activity markedly influenced the transesterification rate but did not modify the enantioselectivity of the two enzymes.

引用

页码：169 / 174

页数：6

共 22 条

[1] ON THE IMPORTANCE OF THE SUPPORT MATERIAL FOR ENZYMATIC-SYNTHESIS IN ORGANIC MEDIA - SUPPORT EFFECTS AT CONTROLLED WATER ACTIVITY [J].

ADLERCREUTZ, P .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 199 (03) :609-614

[2] RESOLUTION OF (+/-)-TRANS-SOBREROL BY LIPASE PS-CATALYZED TRANSESTERIFICATION AND EFFECTS OF ORGANIC-SOLVENTS ON ENANTIOSELECTIVITY [J].

BOVARA, R ;

CARREA, G ;

FERRARA, L ;

RIVA, S .

TETRAHEDRON-ASYMMETRY, 1991, 2 (09) :931-938

[3] PREPARATIVE PRODUCTION OF OPTICALLY-ACTIVE ESTERS AND ALCOHOLS USING ESTERASE-CATALYZED STEREOSPECIFIC TRANS-ESTERIFICATION IN ORGANIC MEDIA [J].

CAMBOU, B ;

KLIBANOV, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (09) :2687-2692

[4] GENERAL-ASPECTS AND OPTIMIZATION OF ENANTIOSELECTIVE BIOCATALYSIS IN ORGANIC-SOLVENTS - THE USE OF LIPASES [J].

CHEN, CS ;

SIH, CJ .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1989, 28 (06) :695-707

[5] CHEMOENZYMATIC SYNTHESIS OF CHIRAL ISOXAZOLE DERIVATIVES [J].

DEAMICI, M ;

DEMICHELI, C ;

CARREA, G ;

SPEZIA, S .

JOURNAL OF ORGANIC CHEMISTRY, 1989, 54 (11) :2646-2650

[6] HOW CAN THE SOLVENT AFFECT ENZYME ENANTIOSELECTIVITY [J].

FITZPATRICK, PA ;

KLIBANOV, AM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (08) :3166-3171

[7] LIPASE-CATALYZED ESTER EXCHANGE-REACTIONS IN ORGANIC MEDIA WITH CONTROLLED HUMIDITY [J].

GODERIS, HL ;

AMPE, G ;

FEYTEN, MP ;

FOUWE, BL ;

GUFFENS, WM ;

VANCAUWENBERGH, SM ;

TOBBACK, PP .

BIOTECHNOLOGY AND BIOENGINEERING, 1987, 30 (02) :258-266

[8] HUMIDITY FIXED-POINTS OF BINARY SATURATED AQUEOUS-SOLUTIONS [J].

GREENSPAN, L .

JOURNAL OF RESEARCH OF THE NATIONAL BUREAU OF STANDARDS SECTION A-PHYSICS AND CHEMISTRY, 1977, 81 (01) :89-96

[9] TEMPERATURE AS AN ENANTIOSELECTIVE PARAMETER IN ENZYMATIC RESOLUTIONS OF RACEMIC MIXTURES [J].

HOLMBERG, E ;

HULT, K .

BIOTECHNOLOGY LETTERS, 1991, 13 (05) :323-326

[10]

Holmberg E., 1990, BIOCATALYSIS, V3, P243

← 1 2 3 →