INSULIN REGULATION OF THE GLUCAGON GENE IS MEDIATED BY AN INSULIN-RESPONSIVE DNA ELEMENT

被引：111

作者：

PHILIPPE, J ^{[1
]}

机构：

[1] CTR MED UNIV GENEVA,DEPT MED,CH-1211 GENEVA 4,SWITZERLAND

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1991年 / 88卷 / 16期

关键词：

GENE REGULATION; GENE TRANSCRIPTION;

D O I：

10.1073/pnas.88.16.7224

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Diabetes mellitus is characterized by insulin deficiency and high plasma glucagon levels, which can be normalized by insulin replacement. It has previously been reported that glucagon gene expression is negatively regulated by insulin at the transcriptional level. By transfection studies, I have now localized a DNA control element that mediates insulin effects on glucagon gene transcription. This element also confers insulin responsiveness to a heterologous promoter. DNA-binding proteins that specifically interact with this insulin-responsive element are found in both glucagon- and nonglucagon-producing cells; and the pattern of binding, as assessed by the gel retardation assay, is not modified by prior insulin treatment.

引用

页码：7224 / 7227

页数：4

共 27 条

[1]

BLACKSHEAR PJ, 1989, CLIN RES, V37, P15

[2] MOLECULAR AND CELLULAR-RESPONSES OF ISLETS DURING PERTURBATIONS OF GLUCOSE-HOMEOSTASIS DETERMINED BY INSITU HYBRIDIZATION HISTOCHEMISTRY [J].

CHEN, L ;

KOMIYA, I ;

INMAN, L ;

MCCORKLE, K ;

ALAM, T ;

UNGER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (04) :1367-1371

[3] ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].

DIGNAM, JD ;

LEBOVITZ, RM ;

ROEDER, RG .

NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489

[4] PROGLUCAGON GENE-EXPRESSION AND POSTTRANSLATIONAL PROCESSING IN A HAMSTER ISLET CELL-LINE [J].

DRUCKER, DJ ;

PHILIPPE, J ;

MOJSOV, S .

ENDOCRINOLOGY, 1988, 123 (04) :1861-1867

[5] EXPRESSION OF A HUMAN PLACENTAL ALKALINE-PHOSPHATASE GENE IN TRANSFECTED CELLS - USE AS A REPORTER FOR STUDIES OF GENE-EXPRESSION [J].

HENTHORN, P ;

ZERVOS, P ;

RADUCHA, M ;

HARRIS, H ;

KADESCH, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (17) :6342-6346

[6] THE INSULIN-RECEPTOR AND THE MOLECULAR MECHANISM OF INSULIN ACTION [J].

KAHN, CR ;

WHITE, MF .

JOURNAL OF CLINICAL INVESTIGATION, 1988, 82 (04) :1151-1156

[7] REGULATION OF AMYLASE GENE-EXPRESSION IN DIABETIC MICE IS MEDIATED BY A CIS-ACTING UPSTREAM ELEMENT CLOSE TO THE PANCREAS-SPECIFIC ENHANCER [J].

KELLER, SA ;

ROSENBERG, MP ;

JOHNSON, TM ;

HOWARD, G ;

MEISLER, MH .

GENES & DEVELOPMENT, 1990, 4 (08) :1316-1321

[8]

KNEPEL W, 1990, J BIOL CHEM, V265, P8725

[9] INSULIN ENHANCES TRANSCRIPTION OF THE TYROSINE AMINOTRANSFERASE GENE IN RAT-LIVER [J].

LEE, KL ;

ISHAM, KR ;

JOHNSON, A ;

KENNEY, FT .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1986, 248 (02) :597-603

[10] DNA-DEPENDENT TRANSCRIPTION OF ADENOVIRUS GENES IN A SOLUBLE WHOLE-CELL EXTRACT [J].

MANLEY, JL ;

FIRE, A ;

CANO, A ;

SHARP, PA ;

GEFTER, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (07) :3855-3859

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