CHARACTERIZATION OF A DNA PROBE FOR DETECTION OF MYCOBACTERIUM-TUBERCULOSIS COMPLEX IN CLINICAL-SAMPLES BY POLYMERASE CHAIN-REACTION

被引：31

作者：

ALTAMIRANO, M ^{[1
]}

KELLY, MT ^{[1
]}

WONG, A ^{[1
]}

BESSUILLE, ET ^{[1
]}

BLACK, WA ^{[1
]}

SMITH, JA ^{[1
]}

机构：

[1] BRITISH COLUMBIA CTR DIS CONTROL, VANCOUVER V5Z 1L8, BC, CANADA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1992年 / 30卷 / 08期

关键词：

D O I：

10.1128/JCM.30.8.2173-2176.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We cloned and sequenced a DNA fragment from Mycobacterium tuberculosis for use in the identification of members of the M. tuberculosis complex. The DNA probe for culture confirmation had a sensitivity and a specificity of 100%. By using primers developed from this probe, the polymerase chain reaction detected 20 mycobacteria by ethidium bromide staining. This polymerase chain reaction system demonstrated 98% sensitivity and 100% specificity for detection of the M. tuberculosis complex in 200 sputum specimens.

引用

页码：2173 / 2176

页数：4

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