PURIFICATION OF PLASMINOGEN FROM HUMAN GRANULOCYTES USING DEAE-SEPHADEX COLUMN CHROMATOGRAPHY

被引：24

作者：

PROKOPOWICZ, J

机构：

[1] Department of Physiological Chemistry, Medical School, Bialystok

[2] Institute for Thrombosis Research, Rikshospitalet, University Hospital, Oslo

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1968年 / 154卷 / 01期

关键词：

D O I：

10.1016/0005-2795(68)90262-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The method of Robbins and Summaria was applied to the purification of plasminogen from human granulocytes. The preparation was 60-fold purified and free from spontaneous fibrinolytic activity. Plasminogen activity was localized in a sharp peak containing 14% of the total protein. A quite similar distribution of plasminogen activity was found by fractionation of human plasma. Cellulose acetate electrophoresis of granulocyte plasminogen preparation showed two fractions. On the basis of our experiments it seems that plasma plasminogen may be derived from granulocytes. © 1968.

引用

页码：91 / +

页数：1

共 16 条

[1] PURIFICATION + PROPERTIES OF HUMAN PLASMINOGEN [J].