CLONING, FUNCTIONAL EXPRESSION AND TISSUE DISTRIBUTION OF HUMAN ALPHA(1C)-ADRENOCEPTOR SPLICE VARIANTS

被引：86
作者：
HIRASAWA, A
SHIBATA, K
HORIE, K
TAKEI, Y
OBIKA, K
TANAKA, T
MURAMOTO, N
TAKAGAKI, K
YANO, J
TSUJIMOTO, G
机构：
[1] NATL CHILDRENS MED RES CTR,DEPT MOLEC CELL PHARMACOL,SETAGAYA KU,TOKYO 154,JAPAN
[2] NIPPON SHINYAKU CO LTD,RES LABS,DEPT MOLEC BIOL,MINAMI KU,KYOTO 601,JAPAN
来源：
FEBS LETTERS | 1995年 / 363卷 / 03期
关键词：
ALPHA(1)-ADRENOCEPTOR; SPLICE VARIANT; CLONING; TISSUE DISTRIBUTION;
D O I：
10.1016/0014-5793(95)00330-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the cloning and characterization of two isoforms of human alpha(1c)adrenoceptor cDNA (alpha(1c)-2, alpha(1c)-3). These isoforms are generated by alternative splicing and differ from the clone we previously isolated (alpha(1c)-1) in their length and sequences of the C-terminal domain. Tissue distribution of mRNAs showed that these variants co-express with alpha(1c)-1 in the human heart, liver, cerebellum and cerebrum. Despite the structural differences, functional experiments in transfected CHO cells showed that the three isoforms have similar ligand binding properties, and all couple with phospholipase C/Ca2+ signaling pathway.
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页码：256 / 260
页数：5
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