AMINO-ACID-METABOLISM BY HEPATOCYTES IN A HYBRID LIVER SUPPORT BIOREACTOR

被引：11

作者：

FUCHS, M

GERLACH, J

ENCKE, J

UNGER, J

SMITH, M

NEUHAUS, P

RIEDEL, E

机构：

[1] FREE UNIV BERLIN,INST BIOCHEM,D-12203 BERLIN,GERMANY

[2] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,CHIRURG KLIN,W-1000 BERLIN,GERMANY

[3] UNIV STRATHCLYDE,BIOENGN UNIT,GLASGOW G1 1XW,LANARK,SCOTLAND

来源：

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS | 1994年 / 17卷 / 12期

关键词：

AMINO ACIDS; FLUORESCENCE HPLC; PIG HEPATOCYTES; CELL PERFUSION SYSTEMS; BIOREACTORS; HYBRID LIVER SUPPORT;

D O I：

10.1177/039139889401701208

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The amino acid patterns of medium perfusate in a liver cell bioreactor developed for a hybrid liver support system have been measured. There were considerable changes in the concentrations of glutamic acid, glutamine, alanine, arginine, ornithine and branched chain amino acids during the first 10 days which is indicative of dynamic cellular metabolism. From day 15, steady state conditions of nitrogen metabolism are reflected by stable amino acid turnover. Monitoring of urea, K+, and P-450 activity suggests that hepatocytes have switched to a stable protein synthesis with a general amino acid uptake and keto acid release following cell volume increase.

引用

页码：663 / 669

页数：7

共 17 条

[1]

BEINERT WD, 1992, GIT FACHZ LAB, V10, P1018

[2] ACCELERATION OF GLUCONEOGENESIS FROM LACTATE BY LYSINE [J].

CORNELL, NW ;

LUND, P ;

HEMS, R ;

KREBS, HA .

BIOCHEMICAL JOURNAL, 1973, 134 (02) :671-672

[3]

FETIG P, 1975, ANN REV BIOCH, V44, P905

[4] ALPHA-KETO ACID METABOLISM BY HEPATOCYTES CULTURED IN A HYBRID LIVER SUPPORT BIOREACTOR [J].

FUCHS, M ;

GERLACH, J ;

UNGER, J ;

ENCKE, J ;

SMITH, M ;

NEUHAUS, P ;

NUNDEL, M ;

RIEDEL, E .

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 1994, 17 (10) :554-558

[5]

FUCHS M, 1994, 12TH DAN S NEPHR 16T

[6]

GERLACH J, 1993, ARTIF ORGANS, V17, P950

[7]

GERLACH J, 1993, TRANSPLANT P, V25, P1593

[8]

GERLACH J, 1994, TRANSPLANTATIONSMEDI, V8, P162

[9]

GERLACH J, 1994, INT J ARTIF ORGANS, V12, P301

[10] NONENZYMATIC VERSUS ENZYMATIC HEPATOCYTE ISOLATION FROM PIG LIVERS FOR LARGER SCALE INVESTIGATIONS OF LIVER-CELL PERFUSION SYSTEMS [J].

GERLACH, JC ;

BROMBACHER, J ;

COURTNEY, JM ;

NEUHAUS, P .

INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS, 1993, 16 (09) :677-681

← 1 2 →