SOUTHERN HYBRIDIZATION ANALYSIS OF THE MECA DELETION FROM METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS

被引：43
作者：
WADA, A
KATAYAMA, Y
HIRAMATSU, K
YOKOTA, T
机构：
[1] JUNTENDO UNIV, FAC MED, DEPT MICROBIOL, 2-1-1 HONGO, BUNKYO KU, TOKYO 113, JAPAN
[2] JUNTENDO UNIV, FAC MED, DEPT BACTERIOL, TOKYO 113, JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 03期
关键词：
D O I：
10.1016/0006-291X(91)90430-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Genomic organization of methicillin-resistant Staphylococcus aureus strains and their methicillin-susceptible subclones were analyzed by pulsed-field gel electrophoresis and Southern hybridization with DNA fragments of methicillin-resistance gene mecA and an insertion element IS431 as probes. The entire mecA gene was deleted in all the seven methicillin-susceptible subclones studied, and the size of the deletion varied from 20 to 100 kilobases depending on each subclone. In six of the seven subclones, however, the downstream deletion end points were confined within a 2.0 kilobase HindIII-HindIII fragment containing a part of IS431 which was located 2.6 kilobase downstream of mecA gene. The results indicated that the intramolecular transposition of IS431 is responsible for the mecA deletion in methicillin-resistant Staphylococcus aureus. © 1991.
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收藏
页码：1319 / 1325
页数：7
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