IDENTIFICATION OF CHEMICAL GROUPS INVOLVED IN BINDING OF PERIODATE-OXIDIZED NADP+ TO 6-PHOSPHOGLUCONATE DEHYDROGENASE

被引：43

作者：

DALLOCCHIO, F ^{[1
]}

NEGRINI, R ^{[1
]}

SIGNORINI, M ^{[1
]}

RIPPA, M ^{[1
]}

机构：

[1] UNIV FERRARA, IST CHIM BIOL, FERRARA, ITALY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1976年 / 429卷 / 03期

关键词：

D O I：

10.1016/0005-2744(76)90312-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Periodate-oxidized NADP+ binds specifically and reversibly to the NADP+ binding site of 6-phosphogluconate dehydrogenase (EC 1.1.1.44) from Candida utilis. The inhibition can be stabilized by reduction with sodium borohydride. An aldehydic group of the inhibitor forms a Schiff base with a lysine residue of the enzyme.

引用

页码：629 / 634

页数：6