A FLUORESCENCE-BASED FIBER OPTIC BIOSENSOR FOR THE FLOW-INJECTION ANALYSIS OF PENICILLIN

被引：24
作者：
XIE, XF
SULEIMAN, AA
GUILBAULT, GG
机构：
[1] Department of Chemistry, University of New Orleans, New Orleans, Louisiana
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 39卷 / 11期
关键词：
FIBER OPTIC BIOSENSOR; PENICILLIN; PENICILLINASE; IMMOBILIZED ENZYME; FLOW INJECTION ANALYSIS; FLUORESCEIN ISOTHIOCYANATE;
D O I：
10.1002/bit.260391111
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A penicillin fiber optic sensor is described. The sensor is based on co-immobilization of a pH indicator, fluorescein isothiocyanate (FITC), and penicillinase on a preactivated biodyne B membrane attached to the end of a bifurcated optical fiber. The characteristics of the sensor are investigated in conjunction with a flow injection analysis system. The proposed sensor is reversible and responds to penicillin in the concentration range of 1 X 10(-4) to 5 X 10(-2) mol/L. The application of this sensor to penicillin analysis in some pharmaceutical samples is demonstrated.
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收藏
页码：1147 / 1150
页数：4
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