RAT MESENTERIC-ARTERY ENDOTHELIAL-CELLS IN CULTURE SECRETE ET-1

被引：10

作者：

GOMEZSANCHEZ, CE ^{[1
]}

FOECKING, MF ^{[1
]}

FERRIS, MW ^{[1
]}

HIEDA, HS ^{[1
]}

GOMEZSANCHEZ, EP ^{[1
]}

机构：

[1] JAMES A HALEY VET ADM MED CTR,TAMPA,FL 33612

来源：

LIFE SCIENCES | 1990年 / 46卷 / 12期

关键词：

D O I：

10.1016/0024-3205(90)90118-B

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Endothelial cells were harvested by the collagenase perfusion of isolated mesenteric arteries of rats and cultured. An endothelin peptide was detected in the supernatant of these cells by an antibody which recognizes ET-1 but not "rat" endothelin (ET-3). Culture media was extracted using a C-8 solid phase column and subjected to reverse phase HPLC using a system that separates all known endothelins and immunoreactive endothelins measured using another antibody which recognizes all endothelins. The main immunoreactive peak co-eluted with ET-1. We could not detect any ET-2, ET-3 or Vasoactive Intestinal Contractor. A smaller immunoreactive peak of unknown structure that eluted earlier than ET-1 was also detected. In conclusion, rat endothelial cells secrete a peptide of similar chromatographic and immunoreactive properties as ET-1. © 1990.

引用

页码：881 / 884

页数：4

共 10 条

[1]

EDWARDS R, 1983, IMMUNOASSAYS CLIN CH, P139

[2]

HIGHSMITH RF, 1988, RELAXING CONTRACTING, P137

[3] THE HUMAN ENDOTHELIN FAMILY - 3 STRUCTURALLY AND PHARMACOLOGICALLY DISTINCT ISOPEPTIDES PREDICTED BY 3 SEPARATE GENES [J].