GENISTEIN, A SELECTIVE PROTEIN TYROSINE KINASE INHIBITOR, INHIBITS INTERLEUKIN-2 AND LEUKOTRIENE-B4 PRODUCTION FROM HUMAN MONONUCLEAR-CELLS

被引：33
作者：
ATLURU, D
JACKSON, TM
ATLURU, S
机构：
[1] UNIV MICHIGAN,MED CTR,MICHIGAN DIABET RES & TRAINING CTR,ANN ARBOR,MI 48109
[2] UNIV MINNESOTA,DEPT FAMILY PRACTICE,MINNEAPOLIS,MN 55455
来源：
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1991年 / 59卷 / 03期
关键词：
D O I：
10.1016/0090-1229(91)90033-7
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
In this study, genistein, a selective protein tyrosine kinase (PTK) inhibitor, inhibited peripheral blood mononuclear cell (PBMC) proliferation and interleukin-2 production from cultures that were stimulated with phytohemagglutinin (PHA), phorbol 12-myristate 13-acetate (PMA) plus A23187, or PHA plus PMA, and genistein effectively blocked the PHA plus IL-2-induced PBMC proliferation. Further, we also found that genistein inhibited LTB4 production from A23187-stimulated cultures whereas H-7, a PKC inhibitor, had no effect on LTB4 production. Our results suggest that PTK may be necessary for the synthesis of LTB4. © 1991.
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收藏
页码：379 / 387
页数：9
相关论文
共 43 条
[1]  
AKIYAMA T, 1987, J BIOL CHEM, V262, P5592
[2]   CONTROL OF POLYCLONAL IMMUNOGLOBULIN PRODUCTION FROM HUMAN-LYMPHOCYTES BY LEUKOTRIENES - LEUKOTRIENE-B-4 INDUCES AN OKT8(+), RADIOSENSITIVE SUPPRESSOR-CELL FROM RESTING, HUMAN OKT8(-) T-CELLS [J].
ATLURU, D ;
GOODWIN, JS .
JOURNAL OF CLINICAL INVESTIGATION, 1984, 74 (04) :1444-1450
[3]   REGULATION OF MITOGEN-STIMULATED HUMAN T-CELL PROLIFERATION, INTERLEUKIN-2 PRODUCTION, AND INTERLEUKIN-2 RECEPTOR EXPRESSION BY PROTEIN-KINASE-C INHIBITOR, H-7 [J].
ATLURU, D ;
POLAM, S ;
ATLURU, S ;
WOLOSCHAK, GE .
CELLULAR IMMUNOLOGY, 1990, 129 (02) :310-320
[4]  
ATLURU S, 1990, BIOCHEM ARCH, V6, P397
[5]   EVIDENCE THAT GENISTEIN, A PROTEIN-TYROSINE KINASE INHIBITOR, INHIBITS CD28 MONOCLONAL-ANTIBODY-STIMULATED HUMAN T-CELL PROLIFERATION [J].
ATLURU, S ;
ATLURU, D .
TRANSPLANTATION, 1991, 51 (02) :448-450
[6]  
BANIYASH M, 1988, J BIOL CHEM, V263, P9874
[7]   THE PROTEIN-KINASE-C INHIBITOR, H-7, INHIBITS ANTIGEN AND IL-2-INDUCED PROLIFERATION OF MURINE T-CELL LINES [J].
CLARK, RB ;
LOVE, JT ;
SGROI, D ;
LINGENHELD, EG ;
SHAAFI, RI .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1987, 145 (02) :666-672
[8]   INHIBITION OF PROTEIN KINASE-C BY H-7 POTENTIATES THE RELEASE OF OLEIC, LINOLEIC AND ARACHIDONIC ACIDS IN A23187-STIMULATED HUMAN NEUTROPHILS [J].
CONQUER, J ;
MAHADEVAPPA, VG .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 167 (01) :168-173
[9]   THE INFLUENCE OF LIPOXYGENASE INHIBITORS ON THE INVITRO PRODUCTION OF HUMAN LEUKOCYTIC PYROGEN AND LYMPHOCYTE ACTIVATING FACTOR (INTERLEUKIN-1) [J].
DINARELLO, CA ;
BISHAI, I ;
ROSENWASSER, LJ ;
COCEANI, F .
INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1984, 6 (01) :43-50
[10]  
FISHER S, 1984, BIOCHEM BIOPH RES CO, V124, P682
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