EFFECT OF THIOL MODIFICATION ON THE THERMAL-STABILITY OF ERYTHROCYTE-MEMBRANES

被引：0
作者：
JOZWIAK, Z
GACZYNSKA, M
SOSZYNSKI, M
机构：
[1] Department of Thermobiology, Institute of Biophysics, University of Łódź
来源：
JOURNAL OF THERMAL BIOLOGY | 1994年 / 19卷 / 02期
关键词：
ERYTHROCYTES; MEMBRANE PROTEINS; SH REAGENTS; THERMAL STABILITY; HEAT;
D O I：
10.1016/0306-4565(94)90062-0
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
1. The effect of chemical modification of membrane proteins on the heat response of erythrocytes has been studied. 2. Erythrocytes preincubated with N-ethylmaleimide (NEM) or p-chloromercuribenzoicacid (pCMB), followed by heating at temperatures above 44 degrees C, indicated a decreased thermal stability. 3. The action of pCMB is much more toxic to erythrocytes than the effect of NEM. 4. These results suggest that modification of proteins by SH reagents could be responsible for changes in the structural integrity of pig erythrocyte plasma membrane leading to their reduced thermal stability.
引用
收藏
页码：141 / 147
页数：7
相关论文
共 33 条
[1]   EFFECT OF HYPERTHERMIA ON THE PLASMA-MEMBRANE STRUCTURE OF CHINESE-HAMSTER V79 FIBROBLASTS - A QUANTITATIVE FREEZE-FRACTURE STUDY [J].
ARANCIA, G ;
MALORNI, W ;
MARIUTTI, G ;
TROVALUSCI, P .
RADIATION RESEARCH, 1986, 106 (01) :47-55
[2]   PARA-(CHLOROMERCURI)BENZENESULFONATE BINDING BY MEMBRANE-PROTEINS AND THE INHIBITION OF WATER TRANSPORT IN HUMAN-ERYTHROCYTES [J].
BENGA, G ;
POPESCU, O ;
POP, VI ;
HOLMES, RP .
BIOCHEMISTRY, 1986, 25 (07) :1535-1538
[3]   CALORIMETRIC STUDIES OF STRUCTURAL TRANSITIONS OF HUMAN ERYTHROCYTE-MEMBRANE - INVOLVEMENT OF SPECTRIN IN A-TRANSITION [J].
BRANDTS, JF ;
ERICKSON, L ;
LYSKO, K ;
SCHWARTZ, AT ;
TAVERNA, RD .
BIOCHEMISTRY, 1977, 16 (15) :3450-3454
[4]   ERYTHROCYTE-MEMBRANE DEFORMABILITY AND STABILITY - 2 DISTINCT MEMBRANE-PROPERTIES THAT ARE INDEPENDENTLY REGULATED BY SKELETAL PROTEIN ASSOCIATIONS [J].
CHASIS, JA ;
MOHANDAS, N .
JOURNAL OF CELL BIOLOGY, 1986, 103 (02) :343-350
[5]   MORPHOLOGICAL-CHANGES, HEMOLYSIS AND MICROVESICULARIZATION OF HEATED HUMAN-ERYTHROCYTES [J].
COAKLEY, WT ;
BATER, AJ ;
CRUM, LA ;
DEELEY, JOT .
JOURNAL OF THERMAL BIOLOGY, 1979, 4 (01) :85-93
[6]   PREPARATION AND CHEMICAL CHARACTERISTICS OF HEMOGLOBIN-FREE GHOSTS OF HUMAN ERYTHROCYTES [J].
DODGE, JT ;
HANAHAN, DJ ;
MITCHELL, C .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1963, 100 (01) :119-&
[7]   TISSUE SULFHYDRYL GROUPS [J].
ELLMAN, GL .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1959, 82 (01) :70-77
[8]   THERMAL-DENATURATION OF THE ERYTHROCYTE CYTOSKELETON ALTERS THE MORPHOLOGICAL-CHANGES ASSOCIATED WITH OSMOTIC SWELLING [J].
ESKELINEN, S ;
COAKLEY, WT ;
TILLEY, D .
JOURNAL OF THERMAL BIOLOGY, 1985, 10 (04) :187-190
[9]   INTRAMOLECULAR AND INTERMOLECULAR CROSS-LINKING OF MEMBRANE PROTEINS IN INTACT ERYTHROCYTES AND GHOSTS BY OXIDIZING AGENTS [J].
HAEST, CWM ;
KAMP, D ;
PLASA, G ;
DEUTICKE, B .
BIOCHIMICA ET BIOPHYSICA ACTA, 1977, 469 (02) :226-230
[10]  
HAHN GM, 1985, CANCER RES, V45, P4138
1234