A UV-VIS SPECTROPHOTOMETER FOR ABSORBENCY MEASUREMENTS OF WEAKLY TRANSPARENT LIGHT-SCATTERING SAMPLES

被引：6
作者：
SOULIGNAC, JC
LAMOTTE, M
机构：
来源：
APPLIED SPECTROSCOPY | 1987年 / 41卷 / 07期
关键词：
D O I：
10.1366/0003702874447734
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
Absorption measurements by transmission through weakly transparent media present some dificulties with coventional commercial spectrophotometers when both good sensitivity and high resolution are required. We describe a home-made computerized spectrophotometer which has been designed for absorption measurements through poorly transparent highly light scattering solutions. Emphasis is given to the difficulties inherent in this type of measurement. Examples are presented to illustrate the effects of the sample thickness and of the fluorescence, which can substantially affect the measured absorbance.
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页码：1220 / 1226
页数：7
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