A NOVEL ENZYMATIC DECARBOXYLATION PROCEEDS VIA A THIOL ESTER INTERMEDIATE

被引：7

作者：

KAWASAKI, T ^{[1
]}

WATANABE, M ^{[1
]}

OHTA, H ^{[1
]}

机构：

[1] KEIO UNIV, FAC SCI & TECHNOL, DEPT CHEM, KOHOKU KU, YOKOHAMA, KANAGAWA 223, JAPAN

来源：

BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN | 1995年 / 68卷 / 07期

关键词：

D O I：

10.1246/bcsj.68.2017

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It is proposed that arylmalonate decarboxylase (AMDase)-catalyzed decarboxylation proceeds via a thiol ester intermediate. Kinetics and CD spectra indicated that alpha-bromophenylacetate is a competitive inhibitor. TOF mass data indicated that the inhibitor bound with the enzyme through a thiol ester bond which was formed between a cysteine residue of the enzyme and the carboxyl group of the inhibitor. This result was also supported by reactivation of the enzyme by the addition of 2-mercaptoethanol, which is expected to cleave the enzyme-inhibitor bond via nucleophilic attack on the thiol ester linkage.

引用

页码：2017 / 2020

页数：4

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