A NEW MEMBER OF THE CYTOSOLIC O-ACETYLSERINE(THIOL)LYASE GENE FAMILY IN ARABIDOPSIS-THALIANA

被引：56

作者：

BARROSO, C

VEGA, JM

GOTOR, C

机构：

[1] UNIV SEVILLA,FAC QUIM,DEPT BIOQUIM,E-41080 SEVILLE,SPAIN

[2] CSIC,INST BIOQUIM VEGETAL & FOTOSINTESIS,E-41080 SEVILLE,SPAIN

来源：

FEBS LETTERS | 1995年 / 363卷 / 1-2期

关键词：

O-ACETYLSERINE(THIOL)LYASE; TRANSCRIPTIONAL REGULATION; SULFUR DEPRIVATION; ENZYME ACTIVATION; ARABIDOPSIS THALIANA;

D O I：

10.1016/0014-5793(95)00255-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA, Atcys-3A, encoding O-acetylserine(thiol)lyase has been isolated from Arabidopsis thaliana. The deduced peptide sequence showed a high level of similarity with the bacterial counterpart, and a remarkable percentage of identity with other higher plant O-acetylserine(thiol)lyase genes. Sequence comparision and Southern blot analysis suggested that Atcys-3A was a new and different to the previously reported member of the cytosolic gene family in Arabidopsis. The Atcys-3A expression was activated by sulfur limitation, requiring a carbon and nitrogen source for maximal expression, A similar pattern of regulation was observed at the O-acetylserine(thiol)lyase activity level. Northern blot analysis also showed an organ-specific expression of Atcys-3A.

引用

页码：1 / 5

页数：5

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