PROPERTIES OF BOVINE NEPHRITOGENIC ANTIGEN THAT INDUCES ANTI-GBM NEPHRITIS IN RATS AND ITS SIMILARITY TO THE GOODPASTURE ANTIGEN

被引：34

作者：

SADO, Y

KAGAWA, M

NAITO, I

OKIGAKI, T

机构：

[1] SHIGEI MED RES INST,DIV ULTRASTRUCT RES,OKAYAMA 70102,JAPAN

[2] SHIGEI MED RES INST,DIV CELL BIOL,OKAYAMA 70102,JAPAN

来源：

VIRCHOWS ARCHIV B-CELL PATHOLOGY INCLUDING MOLECULAR PATHOLOGY | 1991年 / 60卷 / 05期

关键词：

GLOMERULONEPHRITIS; NEPHRITOGENIC ANTIGEN; GLOMERULAR BASEMENT MEMBRANE; COLLAGEN; NC1;

D O I：

10.1007/BF02899566

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The nephritogenic antigen that induces antiglomerular basement membrane antibody-induced glomerulonephritis (anti-GBM nephritis) in rats was isolated from collagenase-solubilized bovine renal basement membranes. Purification was achieved using antibody-coupled affinity columns which were originally used for the purification of trypsin-solubilized nephritogenic antigen (Sado et al. 1984a). The nephritogenic antigen was a heteropolymer composed of P2 (Mr 28 kDa) and P3 (Mr 30 kDa) polypeptides as monomers and their dimers in sodium-dodecyl-sulfate (SDS) polyacrylamide gel electrophoresis. The P3 polypeptide was considered to be the nephritogenic epitope, since a fraction composed of the P2 polypeptide alone was not nephritogenic. The properties of the nephritogenic epitope were the same as those of the Goodpasture epitope (M2*), which is a noncollagenous domain of the alpha-3 chain of type IV collagen (Butkowski et al. 1985; Saus et al. 1988), indicating that the nephritogenic antigen is the same as the Goodpasture antigen.

引用

页码：345 / 351

页数：7

共 19 条

[1]

BUTKOWSKI RJ, 1987, J BIOL CHEM, V262, P7874

[2]

BUTKOWSKI RJ, 1985, J BIOL CHEM, V260, P3739

[3] GLOMERULONEPHRITIS INDUCED IN SHEEP BY IMMUNIZATION WITH HUMAN GLOMERULAR-BASEMENT-MEMBRANE [J].