MU-OPIATE RECEPTOR - CDNA CLONING AND EXPRESSION

被引:397
作者
WANG, JB
IMAI, YS
EPPLER, CM
GREGOR, P
SPIVAK, CE
UHL, GR
机构
[1] NIDA, ADDICT RES CTR, MOLEC NEUROBIOL BRANCH, BALTIMORE, MD 21224 USA
[2] JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROL, BALTIMORE, MD 21224 USA
[3] JOHNS HOPKINS UNIV, SCH MED, DEPT NEUROSCI, BALTIMORE, MD 21224 USA
[4] AMER CYANAMID CO, DIV AGR RES, PRINCETON, NJ 08543 USA
关键词
D O I
10.1073/pnas.90.21.10230
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Mu opiate receptors recognize morphine with high affinity. A 2.1-kb rat brain cDNA whose predicted translation product displays 63% identity with recently described delta and kappa opiate receptor sequences was identified through polymerase chain reaction and cDNA homology approaches. This cDNA recognizes a 10.5-kb mRNA that is expressed in thalamic neurons. COS-cell expression confers naloxonazine-, Na+-, and GTP-sensitive binding of mu but not delta or kappa opioid ligands. Expressing cells bind morphine, [D-Ala2,N-methyl-Phe4,glyol5]enkephalin (DAMGO), and [D-Ala2,D-Leu5]enkephalin (DADLE) with nanomolar or sub-nanomolar affinities, defining a mu opiate receptor that avidly recognizes analgesic and euphoric opiate drugs and opioid peptides.
引用
收藏
页码:10230 / 10234
页数:5
相关论文
共 65 条
[1]   EVIDENCE FOR THE IMPLICATION OF PHOSPHOINOSITOL SIGNAL TRANSDUCTION IN MU-OPIOID INHIBITION OF DNA-SYNTHESIS [J].
BARG, J ;
BELCHEVA, MM ;
COSCIA, CJ .
JOURNAL OF NEUROCHEMISTRY, 1992, 59 (03) :1145-1152
[3]   THE HUMAN N-FORMYLPEPTIDE RECEPTOR - CHARACTERIZATION OF 2 CDNA ISOLATES AND EVIDENCE FOR A NEW SUBFAMILY OF G-PROTEIN-COUPLED RECEPTORS [J].
BOULAY, F ;
TARDIF, M ;
BROUCHON, L ;
VIGNAIS, P .
BIOCHEMISTRY, 1990, 29 (50) :11123-11133
[4]   PRIMARY STRUCTURE OF THE RAT BETA-2 ADRENERGIC-RECEPTOR GENE [J].
BUCKLAND, PR ;
HILL, RM ;
TIDMARSH, SF ;
MCGUFFIN, P .
NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :682-682
[5]  
CHEN Y, 1993, MOL PHARMACOL, V44, P8
[6]   GUANINE NUCLEOTIDES DIFFERENTIATE AGONIST AND ANTAGONIST INTERACTIONS WITH OPIATE RECEPTORS [J].
CHILDERS, SR ;
SNYDER, SH .
LIFE SCIENCES, 1978, 23 (07) :759-761
[7]  
CLARK JA, 1988, MOL PHARMACOL, V34, P308
[8]  
CLARK JA, 1989, J PHARMACOL EXP THER, V251, P461
[9]  
COHEN SR, 1991, NEUROPHARMACOLOGY, V30, P125
[10]  
CRUCIANI RA, 1987, J PHARMACOL EXP THER, V242, P15