PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) STIMULATES GROWTH-HORMONE RELEASE FROM GH(3) CELLS THROUGH TYPE-II PACAP RECEPTOR

被引：33
作者：
MURAKAMI, Y
KOSHIMURA, K
YAMAUCHI, K
NISHIKI, M
TANAKA, J
FURUYA, H
MIYAKE, T
KATO, Y
机构：
[1] First Division, Department of Medicine, Shimane Medical University, Izumo, 693
来源：
REGULATORY PEPTIDES | 1995年 / 56卷 / 01期
关键词：
PACAP; GH RELEASE; GH(3) CELL; SUPERFUSION; ANTAGONIST;
D O I：
10.1016/0167-0115(95)00003-T
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Effect of pituitary adenylate cyclase activating polypeptide (PACAP) on growth hormone (GH) release from GH, cells was studied in a dynamic superfusion system. PACAP-38 and PACAP-27 stimulated GH release from superfused GH, cells. The stimulatory effect of PACAP-38 was comparable to those of vasoactive intestinal polypeptide (VIP) and PACAP-27 at a concentration of 1 nM, but the duration of action was more prolonged in PACAP-38 than in the other two peptides. PACAP(6-38), a selective antagonist of PACAP, as well as a VIP antagonist blunted the GH release induced by PACAP-38 and VIP. An antagonist of GH-releasing factor (GRF) at a concentration of 1 mu M, however, did not affect the GH release induced by PACAP-38. These findings suggest that PACAP and VIP stimulate GH release from GH, cells through type II PACAP receptor but not through the GRF receptors.
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页码：35 / 40
页数：6
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