EFFECTS OF 5-ALPHA-DIHYDROTESTOSTERONE (DHT) ON THE TRANSCRIPTION OF NM23 AND C-MYC GENES IN HUMAN PROSTATIC LNCAP CELLS

被引：11

作者：

YOSHIMURA, I

WU, JM

CHEN, YP

NG, CY

MALLOUH, C

BACKER, JM

MENDOLA, CE

TAZAKI, H

机构：

[1] NEW YORK MED COLL,DEPT BIOCHEM & MOLEC BIOL,VALHALLA,NY 10595

[2] NEW YORK MED COLL,DEPT UROL,VALHALLA,NY 10595

[3] LEDERLE LABS,PEARL RIVER,NY 10565

[4] RUTGERS STATE UNIV,DEPT BIOL SCI,NEWARK,NJ 07102

[5] KEIO UNIV,DEPT UROL,TOKYO,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 208卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.1381

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proliferation of the androgen-dependent human prostate LNCaP cells was increased by the androgen DHT. Changes in the steady state level of the nm23 and c-myc mRNA in LNCaP cells, with or without 10 nM DHT, showed the nm23 mRNA to change rapidly, beginning to rise after 2 h and reaching its peak by 4 h of DHT treatment. In contrast, increases in the c-myc gene only became apparent after 4 h of treatment. Maximal increase of nm23 mRNA was observed at 10(-9) M DHT. The basal expression of c-myc and nm23 mRNAs was between 30-70% lower in the LNCaP cells, as compared with the androgen-independent PC-3 and JCA-1 human prostatic human carcinoma cells. Thus nm23 may be classified as a member of the early androgen-responsive genes. (C) 1995 Academic Press, Inc.

引用

页码：603 / 609

页数：7

共 29 条

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