SPECIFIC DNA-RNA HYBRID BINDING BY ZINC-FINGER PROTEINS

被引：82
|
作者：
SHI, YG [1 ]
BERG, JM [1 ]
机构：
[1] JOHNS HOPKINS UNIV, SCH MED, DEPT BIOPHYS & BIOPHYS CHEM, BALTIMORE, MD 21205 USA
来源：
SCIENCE | 1995年 / 268卷 / 5208期
关键词：
D O I：
10.1126/science.7536342
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Zinc finger proteins of the Cys(2)His(2) type represent a large class of proteins that have been assumed to function by means of specific interactions with DNA. Experiments motivated by structural characteristics of zinc finger protein-DNA complexes revealed that certain zinc finger proteins bound DNA-RNA hybrids with affinities comparable to or greater than those for DNA duplexes. The interactions between the zinc finger proteins and the DNA-RNA hybrids were dependent on which strand was RNA and were sequence-specific. Thus, interactions with DNA-RNA hybrids should be considered with regard to the biological roles of zinc finger proteins.
引用
收藏
页码：282 / 284
页数：3
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