WESTERN-BLOT-ANALYSIS OF VIRUS-SPECIFIC ANTIBODY-RESPONSES FOR CAPRIPOX AND CONTAGIOUS PUSTULAR DERMATITIS VIRAL-INFECTIONS IN SHEEP

被引：43
作者：
CHAND, P
KITCHING, RP
BLACK, DN
机构：
[1] AFRC,INST ANIM HLTH,PIRBRIGHT LAB,WOKING GU24 0NF,SURREY,ENGLAND
[2] CCS HARYANA AGR UNIV,COLL VET SCI,DEPT VET PATHOL,HISAR 125004,HARYANA,INDIA
来源：
EPIDEMIOLOGY AND INFECTION | 1994年 / 113卷 / 02期
关键词：
D O I：
10.1017/S0950268800051803
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
This paper reports the development and evaluation of serological tests for the differentiation of antibodies in animals infected with capripox and parapox viruses. Agar-gel immunodiffusion tests using sera from sheep with naturally-acquired infections and from sheep experimentally inoculated with orf or capripox viruses showed cross reactions. Virus-specific antibody responses to structural proteins of the viruses were analysed by Western-blot analysis. This analysis readily differentiated the infections as either capripox or contagious pustular dermatitis. The antibody responses to the 32 kDa and 28 kDa proteins of capripoxvirus provided a firm basis for differention.
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页码：377 / 385
页数：9
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