DITHIOTHREITOL LIBERATES NONACID LABILE SULFIDE FROM BRAIN-TISSUE OF H2S-POISONED ANIMALS

被引：48

作者：

WARENYCIA, MW ^{[1
]}

GOODWIN, LR ^{[1
]}

FRANCOM, DM ^{[1
]}

DIEKEN, FP ^{[1
]}

KOMBIAN, SB ^{[1
]}

REIFFENSTEIN, RJ ^{[1
]}

机构：

[1] ALBERTA ENVIRONM CTR,VEGRVILLE T0B 4L0,ALBERTA,CANADA

来源：

ARCHIVES OF TOXICOLOGY | 1990年 / 64卷 / 08期

关键词：

Antidotal strategy; Dithiothreitol; Hydrogen sulfide; Persulfides; Tissue-bound sulfide;

D O I：

10.1007/BF01974693

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Acid-labile sulfide measured by conventional gas dialysis and ion chromatography with electrochemical detection accounts for only a proportion of the total sulfide present in brain tissue after poisoning with NaHS, an H2S precursor. Dithiothreitol (DTT) displaced additional measurable sulfide not detectable by the conventional techniques from NaHS-poisoned brain tissue. Sulfide liberation by DTT was dose-dependent and maximal at higher DTT concentration (10 and 30 mM) and was thought to represent non-acid labile sulfide. Dithiothreitol was also found to be significantly protective against H2S poisoning. Furthermore, in vitro inhibition by sulfide of monoamine oxidase (MAO) was reversed by DTT, thus suggesting a molecular mechanism consistent with known persulfide chemistry. Persulfide formation may thus underlie some aspects of hydrogen sulfide neurotoxicity. The rational development of antidotes for use in H2S poisoning may thus have to be centered on strategies concentrating on known thiol, disulfide and persulfide chemistry. © 1990 Springer-Verlag.

引用

页码：650 / 655

页数：6

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