5 SWI/SNF GENE-PRODUCTS ARE COMPONENTS OF A LARGE MULTISUBUNIT COMPLEX REQUIRED FOR TRANSCRIPTIONAL ENHANCEMENT

被引：355
作者：
PETERSON, CL
DINGWALL, A
SCOTT, MP
机构：
[1] UNIV MASSACHUSETTS, SCH MED, DEPT BIOCHEM & MOLEC BIOL, WORCESTER, MA 01605 USA
[2] STANFORD UNIV, MED CTR, SCH MED, DEPT DEV BIOL, STANFORD, CA 94305 USA
[3] STANFORD UNIV, MED CTR, SCH MED, DEPT GENET, STANFORD, CA 94305 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 08期
关键词：
D O I：
10.1073/pnas.91.8.2905
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The Saccharomyces cerevisiae SW11, SWI2 (SNF2), SWI3, SNFS, and SNF6 gene products play a crucial role in the regulation of transcription. We provide here direct biochemical evidence that all rive SWI/SNF polypeptides are components of a large multisubunit complex. These five polypeptides coelute from a gel-filtration column with an apparent molecular mass of almost-equal-to 2 MDa. The five SWI/SNF polypeptides do not copurify when extracts are prepared from swi- or snf- mutants. We show that SWI/SNF polypeptides also remain associated during an affinity-chromatography step followed by gel filtration. Assembly of the SWI/SNF complex is not disrupted by a mutation in the putative ATP-binding site of SWI2, although this mutation eliminates SWI2 function.
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页码：2905 / 2908
页数：4
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