SEPARATIONS OF COMPOUNDS OF BIOLOGICAL AND ENVIRONMENTAL INTEREST BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

被引：31
作者：
BALCHUNAS, AT
SWAILE, DF
POWELL, AC
SEPANIAK, MJ
机构：
[1] Univ of Tennessee, United States
来源：
SEPARATION SCIENCE AND TECHNOLOGY | 1988年 / 23卷 / 12-13期
关键词：
Amines--Chromatographic Analysis - Amino Acids--Chromatographic Analysis - Chromatographic Analysis - Separation - Solvents;
D O I：
10.1080/01496398808075670
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Important criteria for the effective separation of compounds of biological or environmental interest by micellar electrokinetic capillary chromatography are discussed. Efficiencies of approximately 100,000 plates/meter are achieved in the separations of samples of derivatized amines, aflatoxins, and hydroxy aromatic compounds. Laser fluorometric detection is shown to be capable of detecting subpicogram injected quantities. Organic solvents such as 2-propanol and acetonitrile are added to the aqueous mobile phases normally used to improve the separation of hydrophobic compounds, impart different selectivities, and provide a means for gradient programming. Column diameter is found to influence efficiency, analysis time, and detection.
引用
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页码：1891 / 1904
页数：14
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