QUANTITATIVE INVITRO ASSAY FOR HUMAN-IMMUNODEFICIENCY-VIRUS DEOXYRIBONUCLEIC-ACID INTEGRATION

被引：21

作者：

CARTEAU, S ^{[1
]}

MOUSCADET, JF ^{[1
]}

GOULAOUIC, H ^{[1
]}

SUBRA, F ^{[1
]}

AUCLAIR, C ^{[1
]}

机构：

[1] INST GUSTAVE ROUSSY,CNRS,URA 147,PHARMACOL MOLEC,INSERM,U140,RUE CAMILLE DESMOULINS,F-94801 VILLEJUIF,FRANCE

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1993年 / 300卷 / 02期

关键词：

D O I：

10.1006/abbi.1993.1105

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An obligatory step of retroviral growth is the integration of a DNA copy of the viral RNA into the genomic DNA of the host. Recombinant human immunodeficiency virus type I (HIV-1) integrase (IN) expressed in Escherichia coli efficiently catalyzes the overall in vitro integration reaction, namely, the processing of the long terminal repeat (LTR) ends and the strand transfer reaction. Using the 3′ end of synthetic oligonucleotides which match the termini of HIV-1 LTRs as substrate and supercoiled pSP65 DNA as the target, we describe an assay that is suitable for the enzymatic analysis of the integration and for testing candidate inhibitors of HIV IN protein. © 1993 Academic Press, Inc.

引用

页码：756 / 760

页数：5

共 14 条

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[12] HUMAN-IMMUNODEFICIENCY-VIRUS INTEGRATION PROTEIN EXPRESSED IN ESCHERICHIA-COLI POSSESSES SELECTIVE DNA CLEAVING ACTIVITY [J].