HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF AMITRIPTYLINE AND ITS MAIN METABOLITES USING A SILICA COLUMN WITH REVERSED-PHASE ELUENT - APPLICATION IN MICE

被引:15
作者
COUDORE, F
ARDID, D
ESCHALIER, A
LAVARENNE, J
FIALIP, J
机构
[1] FAC MED CLERMONT FERRAND,INSERM,U195,PHARMACOL MED LAB,BP 38,F-63001 CLERMONT FERRAND,FRANCE
[2] FAC PHARM CLERMONT FERRAND,PHARMACOL & PHARM CLIN LAB,F-63001 CLERMONT FERRAND,FRANCE
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 584卷 / 02期
关键词
D O I
10.1016/0378-4347(92)80582-B
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method was developed for the assay of amitriptyline, amitriptyline-N-oxide, nortriptyline, desmethylnortriptyline and E (trans) and Z (cis) isomers of 10-hydroxynortriptyline and of 10-hydroxynortriptyline in plasma and brain of animals, using high-performance liquid chromatography with ultraviolet detection (254 nm). Single extraction was performed at pH 10.5 from 0.25 ml of plasma or 1 ml of brain mixture. Chromatographic separations were achieved with a silica column and an aqueous methanol mobile phase containing ammonia. This procedure offers high sensitivity (8-10 ng/ml), high linearity (r > 0.99) and acceptable precision (coefficient of variation less-than-or-equal-to 13.3%). The method was used to determine levels of amitriptyline and its major metabolites in mice 30 min after a single intraperitoneal administration of amitriptyline (20 mg/kg).
引用
收藏
页码:249 / 255
页数:7
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