THE PRODUCTS OF THE MDR1A AND MDR1B GENES FROM MULTIDRUG RESISTANT MURINE CELLS HAVE SIMILAR DEGRADATION RATES

被引：24

作者：

COHEN, D ^{[1
]}

YANG, CPH ^{[1
]}

HORWITZ, SB ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT CELL BIOL,BRONX,NY 10461

来源：

LIFE SCIENCES | 1990年 / 46卷 / 07期

关键词：

D O I：

10.1016/0024-3205(90)90004-B

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Two vinblastine-resistant sublines of the murine macrophage-like cell line J774.2, J7.V1-1 and J7.V3-1, overproduce unique forms of P-glycoprotein that are encoded by distinct mdr genes, mdrlb and mdrla, respectively. Degradation rates of the two P-glycoprotein isoforms were measured by immunoprecipitation of P-glycoprotein. The half-life of immunoprecipitable P-glycoprotein from J7.V1-1 cells was 16.8 ± 0.5 hours and from J7.V3-1 cells, 17.4 ± 0.5 hours. This rate was not influenced by the presence of vinblastine in the growth medium. The data indicate that P-glycoproteins derived from distinct genes have similar degradation rates. © 1990.

引用

页码：489 / 495

页数：7

共 14 条

[1] INVIVO HALF-LIFE OF A PROTEIN IS A FUNCTION OF ITS AMINO-TERMINAL RESIDUE [J].

BACHMAIR, A ;

FINLEY, D ;

VARSHAVSKY, A .

SCIENCE, 1986, 234 (4773) :179-186

[2] THE 3 MOUSE MULTIDRUG RESISTANCE (MDR) GENES ARE EXPRESSED IN A TISSUE-SPECIFIC MANNER IN NORMAL MOUSE-TISSUES [J].

CROOP, JM ;

RAYMOND, M ;

HABER, D ;

DEVAULT, A ;

ARCECI, RJ ;

GROS, P ;

HOUSMAN, DE .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (03) :1346-1350

[3] THE BIOCHEMISTRY OF P-GLYCOPROTEIN-MEDIATED MULTIDRUG RESISTANCE [J].

ENDICOTT, JA ;

LING, V .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :137-171

[4]

GREENBERGER LM, 1987, J BIOL CHEM, V262, P13685

[5] DISTINCT P-GLYCOPROTEIN PRECURSORS ARE OVERPRODUCED IN INDEPENDENTLY ISOLATED DRUG-RESISTANT CELL-LINES [J].

GREENBERGER, LM ;

LOTHSTEIN, L ;

WILLIAMS, SS ;

HORWITZ, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) :3762-3766

[6]

HSU SIH, 1989, J BIOL CHEM, V264, P12053

[7]

LOTHSTEIN L, 1989, J BIOL CHEM, V264, P16054

[8] ULTRASTRUCTURAL EFFECTS OF COLCHICINE, VINBLASTINE, AND TAXOL IN DRUG-SENSITIVE AND MULTIDRUG-RESISTANT J 774.2 CELLS [J].

MASUROVSKY, EB ;

HORWITZ, SB .

PROTOPLASMA, 1989, 148 (2-3) :138-149

[9] INTERNALIZATION AND DEGRADATION OF MACROPHAGE FC-RECEPTORS DURING RECEPTOR-MEDIATED PHAGOCYTOSIS [J].

MELLMAN, IS ;

PLUTNER, H ;

STEINMAN, RM ;

UNKELESS, JC ;

COHN, ZA .

JOURNAL OF CELL BIOLOGY, 1983, 96 (03) :887-895

[10] STABILITY AND COVALENT MODIFICATION OF P-GLYCOPROTEIN IN MULTIDRUG-RESISTANT KB CELLS [J].

RICHERT, ND ;

ALDWIN, L ;

NITECKI, D ;

GOTTESMAN, MM ;

PASTAN, I .

BIOCHEMISTRY, 1988, 27 (20) :7607-7613

← 1 2 →