A MONOCLONAL-ANTIBODY AGAINST A SYNTHETIC PEPTIDE IS SPECIFIC FOR THE FREE NATIVE HUMAN CHORIONIC-GONADOTROPIN BETA-SUBUNIT

被引：25
作者：
BELLET, D
BIDART, JM
JOLIVET, M
TARTAR, A
CAILLAUD, JM
OZTURK, M
STRUGO, MC
AUDIBERT, F
GRASMASSE, H
ASSICOT, M
BOHUON, C
机构：
[1] INST GUSTAVE ROUSSY, DEPT BIOL CLIN, RUE CAMILLE DESMOULINS, F-94805 VILLEJUIF, FRANCE
[2] INST GUSTAVE ROUSSY, DEPT HISTOPATHOL, F-94805 VILLEJUIF, FRANCE
[3] INST PASTEUR, IMMUNOTHERAPIE EXPTL LAB, F-75724 PARIS 15, FRANCE
[4] INST PASTEUR, CNRS, GR 31, F-75724 PARIS 15, FRANCE
[5] FAC PHARM LILLE, CHIM ORGAN LAB, F-59045 LILLE, FRANCE
来源：
ENDOCRINOLOGY | 1984年 / 115卷 / 01期
关键词：
D O I：
10.1210/endo-115-1-330
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A totally synthetic molecule (109-145 peptide) analogous to the .beta.-subunit carboxyl terminus was used as an antigen in the development of antibodies by the hybridoma technique. A monoclonal antibody (702 D7) specifically recognized the free native .beta.-human chorionic gonadotropin (.beta.hCG). 702 D7 was of the IgG1 subclass and was directed against an antigenic site localized in a 10-amino acid sequence (109-118) or less. The recognition of an epitope located in the 109-118 region could explain the specific recognition of .beta.hCG observed with 702 D7, in contrast to monoclonal antibodies directed against a 118-145 region with a recognition of both .beta.hCG and whole hCG, as observed with a 2nd monoclonal antibody (1032) to synthetic peptide. Immunohistochemical results and preliminary data obtained from the immunoradiometric assay show that 702 D7 provides a clinical tool for the detection of free .beta.-subunit secretion even at low concentrations, and could allow the study of this subunit or its metabolites produced by normal and tumoral cells.
引用
收藏
页码：330 / 336
页数：7
相关论文
共 25 条
[1]  
ARM S, 1983, J CLIN INVEST, V71, P329
[2]   SUCCESSFUL IMMUNIZATION WITH A TOTALLY SYNTHETIC DIPHTHERIA VACCINE [J].
AUDIBERT, F ;
JOLIVET, M ;
CHEDID, L ;
ARNON, R ;
SELA, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (16) :5042-5046
[3]   LOCALIZATION OF THE BETA-SUBUNIT OF HUMAN CHORIONIC-GONADOTROPIN ON VARIOUS TUMORS [J].
BELLET, D ;
ARRANG, JM ;
CONTESSO, G ;
CAILLAUD, JM ;
BOHUON, C .
EUROPEAN JOURNAL OF CANCER, 1980, 16 (04) :433-&
[4]   PRODUCTION AND INVITRO UTILIZATION OF MONOCLONAL-ANTIBODIES TO HUMAN THYROGLOBULIN [J].
BELLET, D ;
SCHLUMBERGER, M ;
BIDART, JM ;
ASSICOT, M ;
CAILLOU, B ;
MOTTE, P ;
VIGNAL, A ;
BOHUON, C .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1983, 56 (03) :530-533
[5]  
BELLET D, 1982, HYBRIDOMA, V1, P218
[6]   PREPARATION AND CHARACTERIZATION OF AN IMPROVED BETA-COOH-TERMINAL IMMUNOGEN FOR GENERATION OF SPECIFIC AND SENSITIVE ANTISERA TO HUMAN CHORIONIC-GONADOTROPIN [J].
BIRKEN, S ;
CANFIELD, R ;
AGOSTO, G ;
LEWIS, J .
ENDOCRINOLOGY, 1982, 110 (05) :1555-1563
[7]  
BIRKEN S, 1977, J BIOL CHEM, V252, P5386
[8]   HUMAN CHORIONIC-GONADOTROPIN BETA-SUBUNIT IS ENCODED BY AT LEAST EIGHT GENES ARRANGED IN TANDEM AND INVERTED PAIRS [J].
BOORSTEIN, WR ;
VAMVAKOPOULOS, NC ;
FIDDES, JC .
NATURE, 1982, 300 (5891) :419-422
[9]   EVIDENCE FOR A GONADOTROPIN FROM NONPREGNANT SUBJECTS THAT HAS PHYSICAL, IMMUNOLOGICAL, AND BIOLOGICAL SIMILARITIES TO HUMAN CHORIONIC-GONADOTROPIN [J].
CHEN, H ;
HODGEN, GD ;
MATSUURA, S ;
LIN, LJ ;
GROSS, E ;
REICHERT, LE ;
BIRKEN, S ;
CANFIELD, RE ;
ROSS, GT .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (08) :2885-2889
[10]   ABSENCE OF THE COOH-TERMINAL PEPTIDE ON ECTOPIC HUMAN CHORIONIC-GONADOTROPIN BETA-SUBUNIT (HCGE) [J].
COLE, LA ;
BIRKEN, S ;
SUTPHEN, S ;
HUSSA, RO ;
PATTILLO, RA .
ENDOCRINOLOGY, 1982, 110 (06) :2198-2200
123