A CA2+ CHANNEL IN RENAL EPITHELIAL-CELLS INTRODUCED BY PARATHYROID-HORMONE

被引：0
作者：
SUZUKI, M
TANIGUCHI, J
机构：
来源：
MINERAL AND ELECTROLYTE METABOLISM | 1995年 / 21卷 / 1-3期
关键词：
CATION CHANNEL; FURA-2; PATCH CLAMP;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
To investigate the mechanism of Ca2+ influx after hormonal stimuli, patch clamping with [Ca2+](i) fluorescence measurement was performed in single renal proximal epithelial cells. A Ca2+ current appeared along with a rise in [Ca2+](i) during parathyroid hormone addition to the bath, but not when the hormone was inside the cell-attached pipette. The Ca2+ channel, possessing linear I-V, was nonselective for cations, most active in hyperpolarized -membranes and showed oscillatory fluctuations in opening, which disappeared after excision. GTP gamma S opened the channel that had disappeared, which was suppressed by GDP beta S. These data show a PTH-responsive Ca2+ channel for which GTP/GDP may act as second messenger to open the latent Ca2+ channel.
引用
收藏
页码：35 / 39
页数：5
相关论文
共 20 条
  • [1] A NON-SELECTIVE CATION CONDUCTANCE IN FROG-MUSCLE MEMBRANE BLOCKED BY MICROMOLAR EXTERNAL CALCIUM-IONS
    ALMERS, W
    MCCLESKEY, EW
    PALADE, PT
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1984, 353 (AUG): : 565 - 583
  • [2] NON-SELECTIVE CONDUCTANCE IN CALCIUM CHANNELS OF FROG-MUSCLE - CALCIUM SELECTIVITY IN A SINGLE-FILE PORE
    ALMERS, W
    MCCLESKEY, EW
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1984, 353 (AUG): : 585 - 608
  • [3] ACTIVATION OF FACILITATION CALCIUM CHANNELS IN CHROMAFFIN CELLS BY D1 DOPAMINE-RECEPTORS THROUGH A CAMP PROTEIN KINASE-A-DEPENDENT MECHANISM
    ARTALEJO, CR
    ARIANO, MA
    PERLMAN, RL
    FOX, AP
    [J]. NATURE, 1990, 348 (6298) : 239 - 242
  • [4] ACTIVATION OF LATENT CA-2+ CHANNELS IN RENAL EPITHELIAL-CELLS BY PARATHYROID-HORMONE
    BACSKAI, BJ
    FRIEDMAN, PA
    [J]. NATURE, 1990, 347 (6291) : 388 - 391
  • [5] RUN-DOWN OF THE CA CURRENT DURING LONG WHOLE-CELL RECORDINGS IN GUINEA-PIG HEART-CELLS - ROLE OF PHOSPHORYLATION AND INTRACELLULAR CALCIUM
    BELLES, B
    MALECOT, CO
    HESCHELER, J
    TRAUTWEIN, W
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1988, 411 (04): : 353 - 360
  • [6] BERRIDGE MJ, 1987, ANNU REV BIOCHEM, V56, P159, DOI 10.1146/annurev.bi.56.070187.001111
  • [7] A CALCIUM-PERMEABLE STRETCH-ACTIVATED CATION CHANNEL IN RENAL PROXIMAL TUBULE
    FILIPOVIC, D
    SACKIN, H
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (01): : F119 - F129
  • [8] PURIFIED DIHYDROPYRIDINE-BINDING SITE FROM SKELETAL-MUSCLE T-TUBULES IS A FUNCTIONAL CALCIUM-CHANNEL
    FLOCKERZI, V
    OEKEN, HJ
    HOFMANN, F
    PELZER, D
    CAVALIE, A
    TRAUTWEIN, W
    [J]. NATURE, 1986, 323 (6083) : 66 - 68
  • [9] CYTOPLASMIC CALCIUM IN INDIVIDUAL PROXIMAL TUBULAR CELLS IN CULTURE
    GOLIGORSKY, MS
    LOFTUS, DJ
    HRUSKA, KA
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 251 (05): : F938 - F944
  • [10] DUAL ION-CHANNEL REGULATION BY CYCLIC-GMP AND CYCLIC GMP-DEPENDENT PROTEIN-KINASE
    LIGHT, DB
    CORBIN, JD
    STANTON, BA
    [J]. NATURE, 1990, 344 (6264) : 336 - 339
12